Cell Culture


Cell Culture

Animal and Plant Cell Culture Facilities for Biological and Bioengineering Research

The Cell Culture Facility (CCF) located in the Life Science Laboratories supports research by providing space, equipment, cell cultures (plant and mammalian), media, supplies and training on a recharge basis for both academic labs and corporate partners. The Facility also offers technical expertise and hands on support for custom research projects. Labs are equipped with laminar flow hoods, incubators, centrifuges, refrigerators/freezers, basic microscopes and automated cell counters.

Flasks / Cells

Great ideas, but no lab?  We can do the wet work for you!

  • Cell Line expansion and cryopreservation
  • Stem cell culture
  • Liquid nitrogen storage
  • Assay Development/optimization
  • CRISPR based gene editing and stable clone selection
  • Mycoplasma testing
  • Lentiviral packaging, titer, transduction
  • Molecular biology

Questions about something different? Ask us!
We also offer consultation to help you move your research forward.

PCCL culture

Plant Cell Culture Library (PCCL)

The Plant Cell Culture Library (PCCL) at UMass Amherst is a globally unique collection of callus cell cultures representing more than 1,000 diverse plant species.  Plants produce over 200,000 different kinds of small organic chemicals called “secondary” or “specialized” metabolites that serve many important roles in plant biology, and have been used in various industries including pharmaceuticals, cosmetics, flavors, dyes, dietary supplements and fragrances.

This unique living resource is available to the entire research community to discover new biological pathways or new methods for plant based product development.

Starter cultures and growth media may be purchased from the Collection for academic or commercial research with no IP or licensing restrictions.

Search the Collection at umass.edu/ials/pccl-database.

Labspace and Equipment

Mammalian Cell Culture (LSL S570)

Mammalian Cell Culture

Location: LSL S570

  • 2 BSL2 Biosafety cabinets (1  BSL2+)
  • 2 Heracell VIOS 160i Incubators (1 with low oxygen capability)
  • 2 CellCyte2 Live Cell Imagers with Blue/Green/Red fluorescence
  • Countess FL3 automated cell counter (red/green fluorescence capability)
  • Sorvall ST8 centrifuge with rotors for 15/50mL tubes and Multiwell plates
  • Microcentrifuge
  • Inverted Nikon Ts2 phase contrast microscope
  • Refrigerator
  • -20 Freezer
  • -80 Freezer
  • Liquid nitrogen storage
  • Common consumables and media on re-charge basis 
General Cell Culture (S471)

Mammalian Cell Culture

Location: LSL S471

  • 2 BSL2 Biosafety cabinets 
  • 4 Heracell VIOS 160i Incubators 
  • 2 Inverted Nikon Ts2 / Ts2F phase contrast microscopes 
    • 1 with Green and Red Fluorescence and wifi camera 
  • Megafuge ST4R Plus Centrifuge 
    • 15/50mL Tubes, muliwell plates, aerosol lids, refrigeration, ramp speed control 
  • Countess FL3 automated cell counter (red/green fluorescence capability) 
  • 4D-Nucleofector Core and X units for electroporation 
  • Common consumables and media on re-charge basis 
  • 4°C and -20°C storage 
  • Liquid Nitrogen storage
Plant Cell Culture (LSL S579)

Plant Cell Culture

Location: LSL S579

  • 2 BSL2 Biosafety cabinets
  • Germinator 500 instrument sterilizer

Media prep station, with

  • Barnstead EASYpure water filtration system
  • Analytical balance and bench scale
  • Magnetic stir plates

Staff

    • Location

    Life Science Laboratories
    S471, S560G, S570, S579
    University of Massachusetts Amherst
    240 Thatcher Road
    Amherst, MA 01003