Cell Culture
Cell Culture Facility: This facility is set up for walk-in usage by registered users and maintains common equipment for routine mammalian cell culture work, including: single and multi-channel pipetmen, serological pipetors, water baths, Cold storage (liquid Nitrogen, 4°C, -20°C, -80°C), 7 BSL2 (1, 2+) Biosafety Cabinets, 8 Heracell VIOS 160i CO2 incubators (1w/ O2 regulation capability), 2 Nikon Eclipse Ts2 microscopes, Nikon Eclipse Ts2-FL with 470 and 560nm filter sets and a 12MP camera, Invitrogen Countess 2FL & 3FL Automated Cell Counters, Thermo Scientific Megafuge ST4R Plus-MD centrifuge, TX-1000 rotor with ClickSeal biocontainment lids and adapters for 15 mL/ 50 mL conical tubes and deepwell/microplates, 2 Sorvall ST8 centrifuges with rotors and buckets for micro/ conical tubes, 100 mL bottles and microplates, Lonza 4D-Nucleofector Core with an X unit for cell transfection via electroporation, World Precision Instruments EVOM Manual with an STX4 Electrode for trans-epithelial electrical resistance (TEER) measurements.
Additionally, the Facility stocks common TC consumables and media for users to purchase.
Finally, facility staff offers training and wet lab services, such as lentivirus production/titer, cell line transfection/transduction, stable cell line/clonal generation, assay development support, mycoplasma testing, CRISPR based gene knock-out/knock-in, molecular biology services and general consultation.