Biophysical Characterization

The Odyssey CLx imaging system from LI-COR allows researchers to analyze and quantify up to nine miniblots or six micro-plates in a single scan. Even faint bands can be imaged and analyzed. The resulting images are of publication quality.

Typhoon FLA 9500 is a variable mode laser scanner with modular access to the optical components, providing both versatile and flexible imaging for precise quantitation of proteins, nucleic acids, and other biomolecules.

The Rock Imager 2 has both visible and UV imaging capabilities and can be used as a high-powered microscope to manually explore the contents of your plates for crystals.

The Analytical Ultracentrifuge Fluorescence Detection System (AU-FDS) accessory for the Beckman XL-A / XL-I instruments compliments the existing detectors.

The ProteomeLab XL-I is used for in-solution characterization of proteins, oligomers, aggregates, particles, colloids, and small structures. The analytical ultracentrifuge in the facility can be used for several types of experiments, but the two most widely used are sedimentation velocity and sedimentation equilibrium.

The Synergy 2 is BioTek’s high-performance multi-mode plate reader designed for life science research and drug discovery. Detection modes include Fluorescence Intensity, Fluorescence Polarization, Time-Resolved Fluorescence, AlphaScreen, Luminescence and UV-visible Absorbance.

The Formulator 10 is a liquid handling system that is used to generate formulation combinations in 96 well plates for crystallization experiments.
The NT8 is a nanoliter-volume liquid handler with drop dispensing capability for adding protein to 96 well plates in crystallization experiments.

Circular dichroism (CD) spectroscopy is a powerful technique to probe the secondary and tertiary structures of proteins and peptides. The Jasco J-1500 and the accessories allow researchers to run a wide range of experiments.

The Malvern Auto-iTC200 is a fully automated (cleaning steps, titrations, filling cells, equilibrations, etc.) low volume, highly sensitive isothermal titration calorimeter. Applications include characterizing molecular interactions of small molecules, proteins, antibodies, nucleic acids, lipids and other biomolecules.

The Zetasizer Nano ZSP incorporates three techniques (dynamic and static light scattering, microelectrophoresis) in a single compact instrument. There is a range of options and accessories so researchers can measure: particle and molecule size distributions, zeta potential, and molecular weights.

Microscale Thermophoresis (MST) is a powerful method to quantify biomolecular interactions. It measures the motion of molecules along microscopic temperature gradients and detects changes in their hydration shell, charge or size and translates this into a measure of binding affinity.

Size Exclusion Chromatography-Multi-Angle Light Scattering (SEC-MALS) measures molar mass directly, independent of elution time. As an absolute technique, SEC-MALS is used to characterize such attributes as the aggregation state of proteins and the stoichiometry of complexes.