Biophysical Characterization
Biophysical Characterization Core Facility: Facility comprises equipment for characterization of biomolecular interactions, including MicroCal AutoITC-200 with auto-sampler, Biacore T-200 surface plasmon resonance system, two Nanotemper thermophoresis instruments (one for proteins with extrinsic labels, the other for Trp excitation), a Biotek microplate reader equipped to detect absorbance, fluorescence, luminescence, and anistropy, an Odyssey Li-COR CLx imaging system, GE Typhoon phosphorimager, Beckman analytical ultracentrifuge with Aviv fluorescence detector, Malvern DLS/Zetasizer Nano ZSP system with MPT-2 (Titrator)/Degasser, Agilent SEC-MALS chromatography system with UV, RI and Wyatt multi-angle light scattering detectors, JASCO J-1500 circular dichroism instrument with sample changer and stopped flow accessory. In addition, our protein crystallography facility is equipped with a robotics crystallization set-up to prepare crystallization screens, along with the imaging stations necessary to monitor crystal growth. Finally, we have a Rigaku x-ray source that will enable acquisition of small-angle x-ray scattering data as well as serving for single crystal diffraction.