Histology–Tissue Resources


Histology–Tissue Resources

HistoSpring works with surgeons and pathologists at Baystate Medical Center to acquire tissues needed for research. This facility currently has a bank of over 200 normal breast tissues with associated interview data as well as data and specimens (blood and tissue) from breast cancer patients who are enrolled in the Rays of Hope Breast Research Registry. Occasionally breast tumor specimens are provided and fixed, frozen and processed for organoid culture. The facility also coordinates the collection of ovarian tumor tissues with matching blood. These tumors are also formalin fixed paraffin embedded, snap frozen and frozen in DMSO as organoids. Collections are ongoing to support fresh tissue needs.  Depending on investigator requirements there is potential for specific treatments (i.e. explant cultures) and to expand to several other tissue types.

HistoSpring has the capability to process and paraffin embed human, animal and plant tissues, section fixed or frozen tissues, as well as perform histological analyses.

  • Immunohistochemical analysis using a Dako autoimmunostainer allows for larger batch staining with antibodies in a controlled fashion.  
  • Isolation and analysis of specific cell populations in fixed and frozen tissues using a laser capture micro-dissection.
  • Technical staff is available for all histological tasks as well as providing training.  See “Rates and Services” for pricing information.
Remote video URL
Watch Core Facilities Seminar: Histology-BRaMA on YouTube.
Tuesday, October 20, 2020

Histology-BRaMA

2:00 pm

Sallie Schneider, Director of Biospecimen Resource and Molecular Analysis Facility, Pioneer Valley Life Sciences Institute

Equipment

Sample Prep

  • 2 DAKO auto-immunostainers
  • conventional robotic histology system
  • Leica autostainer
  • Leica autoprocessor
  • Leica CM3050 cryostat

Staff