Flow Cytometry


Flow Cytometry

Imaging Structures Ranging from Single Molecules to Whole Model Organisms

Designed to assist members of the research community at UMass and on the other Five College campuses to conduct research using flow cytometry and live animal imaging technologies. The equipment and services provided by the facility are unmatched on campus and in the five college area with the nearest facility at UMass Medical School in Worcester, more than one hour’s drive from campus.

Image Acknowledgement: We would like to thank Prof. Lisa Minter, VASCI, and Heather Sherman, MCB, for providing the header image.

Watch Core Facilities Seminar Series: Flow Cytometry on YouTube.
Tuesday, April 19, 2022

Flow Cytometry

2:00 pm

Amy Burnside, Director of Flow Cytometry

Guest Speaker: David Luther, (Rotello group) Department of Chemistry–"Quantifying cytosolic protein delivery through imaging flow cytometry."

Equipment

Amnis ImageStream Mark II Imaging Flow Cytometer

Amnis ImageStream Mark II Imaging Flow Cytometer

Combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy.

BD DUAL LSRFortessa

BD DUAL LSRFortessa

Analysis Capabilities for TWO USERS Simultaneously (the third such instrument in the country)

  • Left side – 3 Excitation Lasers: 405nm, 488nm, and 640nm, High Throughput Sampler 15 color analysis capabilities.
  • Right side – 5 Excitation Lasers: 355nm (UV), 405nm, 488nm, 561nm, and 640nm 18 color analysis capabilities.
  • (Optical Configuration Details)
BD FACSAria Fusion

BD FACSAria Fusion

Streamlines sorting and analysis with its fixed alignment flow cell, fixed optical architecture and software-controlled setup and operation.

  • Index sorting capabilities
  • 4 Excitation Lasers-488, 640, 561 and 405-nm (no UV)
  • Class II Type A2 biosafety cabinet
Cytek Aurora

Cytek Aurora

Full spectrum technology allows up to:

  • 40 colors
  • Five lasers
  • 64 fluorescence channels
  • State-of-the-art optics and low-noise electronics
  • View multiple panels simultaneously.

Staff

    • Location

    068 Integrated Sciences Building
    University of Massachusetts Amherst
    661 North Pleasant Street
    Amherst, MA 01003

    (413) 545-2315 (Lab)