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Mass Spectrometry

Mass Spectrometry

The core provides mass spectrometry instrumentation and expertise to serve the life science community in academia and industry. A broad range of capabilities and techniques available. These include proteomics, small molecule and metabolite identification and quantitation, characterization of mixtures by LC-MS, molecular imaging of tissue and other materials, structural and stoichiometry studies using native electrospray, and protein structural/dynamics and ligand-binding using hydrogen/deuterium exchange MS. Instrumentation includes Orbitrap Fusion high resolution UPLC-MS, Waters Synapt with automated HDX robotics, as well as MALDI-TOF/TOF and ICP-MS with molecular imaging capabilities.

Other research groups who make use of the Cores instrumentation are involved in investigation of protein structure and dynamics by hydrogen/deuterium exchange, understanding and tailoring enzyme mechanisms, and also MS method development and fundamental studies in protein/protein and protein/ligand interaction.

Instrumentation encompasses a broad range of analytical capabilities from small molecule analysis to large biopolymers, including GC-MS, HPLC-MS, UPLC and nanoLC chromatography systems.

  • Thermo Orbitrap Fusion This versatile instrument combines high speed with high mass accuracy and versatile fragmentation techniques to provide an extremely powerful instrument for proteomics and metabolomics workflows. Uses include analysis of protein modification, degradation, cross-linking, quantitative proteomics.
  • Waters Synapt G2Si with HDX automation Combining high resolution mass spectrometry with ion mobility separation enables characterization of complex mixtures as well as macromolecular non-covalent complexes for structural elucidation. Automated HDX technology enables hydrogen-deuterium exchange studies to investigate structural dynamics of proteins and complexes or compare ligand binding properties.
  • Bruker ultrafleXtreme MALDI-TOF/TOF MALDI is highly sensitive for detecting low abundance proteins, peptides and small molecules. Rastering capabilities of this instrument enable imaging to localize proteins, metabolites and/or drugs within tissue samples.
  • Perkin-Elmer NeXion ICP-MS Elemental speciation for highly sensitive quantitation combined with laser ablation for tissue imaging.
  • Waters Xevo TQD This powerful workhorse triple quadrupole mass spectrometer enables sensitive quantitation of small molecules. Combined with ultra-high pressure LC system for rapid separations and method development.
  • Agilent 7000C GC triple quad enables separation and quantitation of complex mixtures of volatile materials, e.g. metabolites, natural products, etc.

Other instrumentation in the Mass Spectrometry Center:

  • Bruker SolariX FT-ICR
  • Bruker MicroTOF II ESI
  • Bruker MicroFLEX MALDI
  • JEOL MStation sector with EI and FAB sources
  • AB Sciex QStarXL ESI-TOF
  • Bruker HCT Ultra ETD II ion trap
  Campus Users Other Academic Institutions Industry
Mass Spectrometry Service Analysis
Low Resolution Analysis $40/sample $50/sample $100/sample
High Resolution (Accurate Mass) Analysis $40/sample $50/sample $100/sample
Mass Spectrometry Hourly Usage
Daytime Usage $45/hour - -
Night Usage (6pm-8am) $35/hour - -
Advanced Data Acquisition/Analysis
Staff $100/hour $100/hour $200/hour
Rates are subject to change, contact facility to verify current fees.
Updated March 13, 2017

Facility Online Manager (FOM) (access and reservations)

Facility Staff

Dr. Stephen EylesDr. Stephen Eyles
(413) 577-1528
eyles@biochem.umass.edu

umass.edu/massspec

Location

S540 Life Science Laboratories
University of Massachusetts Amherst
240 Thatcher Road
Amherst, MA 01003

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