Sallie W. Smith-Schneider

Research Interests

Signaling During Mammary Gland Development and Carcinogenesis

Development of the mature mammary gland requires numerous signaling pathways engaged by secreted factors and cell:cell interactions. My overall interest is in studying the signaling pathways that control normal and abnormal development in the mammary gland, as well as the role of communication between various cellular compartments in these processes.

In the past few years we have focused on the role of Notch receptor proteins during normal and abnormal development. The Notch family of proteins is comprised of four extracellular membrane-associated receptors (Notch 1-4) whose structure and function have been highly conserved throughout evolution. Signaling through these receptors is initiated by ligand on adjacent cells, which results in activation through proteolytic cleavage events at the membrane. The cytoplasmic domain is released and translocates to the nucleus where it participates in numerous protein:protein interactions which affect transcription.

Transgenic studies have indicated that deregulated expression of the Notch4 receptor results in a retarded growth of the epithelial cell compartment of the mammary gland and a block in lobulo-alveolar development. Furthermore, these mice develop breast tumors within three to six months. My lab has been studying signaling pathways induced by Notch receptors and how these pathways interact with a number of other signaling pathways involved in mammary gland development. I am particularly interested in the interplay between Notch signaling and steroid receptor transactivation, p53 activation, and activation of epidermal growth factor receptor family members. Activation of Notch receptors in one cell also appears to initiate an interesting set of communicative events with neighboring cells. Secretion of various factors and changes in cell surface receptors appear to modulate the normal responses in other cellular compartments and affect immune surveillance in co-culture assays. These events may be as important as the internal signaling pathways in the turn towards malignant growth.

Publications

Narayanaswamy, V., Pentecost, B. T., Telfer, J. C., Burnside, A. S., Schneider, S. S., Alfandari, D., et al.. (2022). Durable antibody and effector memory T cell responses in breastmilk from women with SARS-CoV-2. Front Immunol, 13, 985226. presented at the 2022. doi:10.3389/fimmu.2022.985226

Narayanaswamy, V., Pentecost, B. T., Schoen, C. N., Alfandari, D., Schneider, S. S., Baker, R., & Arcaro, K. F.. (2022). Neutralizing Antibodies and Cytokines in Breast Milk After Coronavirus Disease 2019 (COVID-19) mRNA Vaccination. Obstet Gynecol, 139(2), 181-191. presented at the 2022 02 01. doi:10.1097/AOG.0000000000004661

Morin, S. M., Majhi, P. Dhangada, Crisi, G. M., Gregory, K. J., Franca, R., Schalet, B., et al.. (2022). Interindividual variation contributes to differential PCB 126 induced gene expression in primary breast epithelial cells and tissues. Ecotoxicol Environ Saf, 241, 113722. presented at the 2022 Aug. doi:10.1016/j.ecoenv.2022.113722

Majhi, P. Dhangada, Griner, N. B., Mayfield, J. A., Compton, S., Kane, J. J., Baptiste, T. A., et al.. (2021). Genetic modifiers regulating DNA replication and double-strand break repair are associated with differences in mammary tumors in mouse models of Li-Fraumeni syndrome. Oncogene, 40(31), 5026-5037. presented at the 2021 08. doi:10.1038/s41388-021-01892-5

Mogus, J. P., LaPlante, C. D., Bansal, R., Matouskova, K., Schneider, B. R., Daniele, E., et al.. (2021). Exposure to Propylparaben During Pregnancy and Lactation Induces Long-Term Alterations to the Mammary Gland in Mice. Endocrinology, 162(6). presented at the 2021 06 01. doi:10.1210/endocr/bqab041

Majhi, P. Dhangada, Sharma, A., Roberts, A. L., Daniele, E., Majewski, A. R., Chuong, L. M., et al.. (2020). Effects of Benzophenone-3 and Propylparaben on Estrogen Receptor–Dependent R-Loops and DNA Damage in Breast Epithelial Cells and Mice. Environmental Health Perspectives, 128, 017002. Retrieved from https://doi.org/10.1289/ehp5221

Gregory, K. J., Morin, S. M., Kubosiak, A., Ser-Dolansky, J., Schalet, B. J., D Jerry, J., & Schneider, S. S.. (2020). The use of patient-derived breast tissue explants to study macrophage polarization and the effects of environmental chemical exposure. Immunol Cell Biol, 98(10), 883-896. presented at the 2020 11. doi:10.1111/imcb.12381

Dunphy, K. A., Black, A. L., Roberts, A. L., Sharma, A., Li, Z., Suresh, S., et al.. (2020). Inter-Individual Variation in Response to Estrogen in Human Breast Explants. J Mammary Gland Biol Neoplasia, 25(1), 51-68. presented at the 2020 03. doi:10.1007/s10911-020-09446-3

Dunphy, K. A., Black, A. L., Roberts, A. L., Sharma, A., Li, Z., Suresh, S., et al.. (2020). Inter-Individual Variation in Response to Estrogen in Human Breast Explants. Journal of Mammary Gland Biology and Neoplasia, 25, 51–68. Retrieved from https://doi.org/10.1007/s10911-020-09446-3

Majhi, P. Dhangada, Sharma, A., Roberts, A. L., Daniele, E., Majewski, A. R., Chuong, L. M., et al.. (2020). Effects of Benzophenone-3 and Propylparaben on Estrogen Receptor-Dependent R-Loops and DNA Damage in Breast Epithelial Cells and Mice. Environ Health Perspect, 128(1), 17002. presented at the 2020 01. doi:10.1289/EHP5221

Muse, M. E., Titus, A. J., Salas, L. A., Wilkins, O. M., Mullen, C., Gregory, K. J., et al.. (2020). Enrichment of CpG island shore region hypermethylation in epigenetic breast field cancerization. Epigenetics, 15, 1093–1106. Retrieved from https://doi.org/10.1080/15592294.2020.1747748

Gregory, K. J., Roberts, A. L., Conlon, E. M., Mayfield, J. A., Hagen, M. J., Crisi, G. M., et al.. (2019). Gene expression signature of atypical breast hyperplasia and regulation by SFRP1. Breast Cancer Research, 21. Retrieved from https://doi.org/10.1186/s13058-019-1157-5

Gregory, K. J., Roberts, A. L., Conlon, E. M., Mayfield, J. A., Hagen, M. J., Crisi, G. M., et al.. (2019). Gene expression signature of atypical breast hyperplasia and regulation by SFRP1. Breast Cancer Res, 21(1), 76. presented at the 2019 06 27. doi:10.1186/s13058-019-1157-5

Williams, K. E., Jawale, R. M., Schneider, S. S., Otis, C. N., Pentecost, B. T., & Arcaro, K. F.. (2018). DNA methylation in breast cancers: Differences based on estrogen receptor status and recurrence. Journal of Cellular Biochemistry. presented at the sep. Retrieved from https://doi.org/10.1002/jcb.27431

D Jerry, J., Shull, J. D., Hadsell, D. L., Rijnkels, M., Dunphy, K. A., Schneider, S. S., et al.. (2018). Genetic variation in sensitivity to estrogens and breast cancer risk. Mamm Genome, 29(1-2), 24-37. presented at the 2018 02. doi:10.1007/s00335-018-9741-z

Zimmers, S. M., Browne, E. P., Williams, K. E., Jawale, R. M., Otis, C. N., Schneider, S. S., & Arcaro, K. F.. (2018). TROP2 methylation and expression in tamoxifen-resistant breast cancer. Cancer Cell International, 18. presented at the jul. Retrieved from https://doi.org/10.1186/s12935-018-0589-9

D Jerry, J., Shull, J. D., Hadsell, D. L., Rijnkels, M., Dunphy, K. A., Schneider, S. S., et al.. (2018). Genetic variation in sensitivity to estrogens and breast cancer risk. Mammalian Genome, 29, 24–37. presented at the feb. Retrieved from https://doi.org/10.1007/s00335-018-9741-z

Schneider, S. S., Henchey, E. M., Sultana, N., Morin, S. M., D Jerry, J., Makari-Judson, G., et al.. (2017). Individual-specific variation in the respiratory activities of HMECs and their bioenergetic response to IGF1 and TNFa. Journal of Cellular Physiology, 232, 2750–2765. presented at the may/2017. Retrieved from https://doi.org/10.1002/jcp.25932

Schneider, S. S., Henchey, E. M., Sultana, N., Morin, S. M., D Jerry, J., Makari-Judson, G., et al.. (2017). Individual-specific variation in the respiratory activities of HMECs and their bioenergetic response to IGF1 and TNFα.. J Cell Physiol, 232(10), 2750-2765. presented at the 2017 Oct. doi:10.1002/jcp.25932

Yang, H. P., Schneider, S. Smith, Chisholm, C. M., Browne, E. P., Mahmood, S., Gierach, G. L., et al.. (2015). Association of TGF-β2 levels in breast milk with severity of breast biopsy diagnosis.. Cancer Causes Control, 26(3), 345-54. presented at the 2015 Mar.

Rotunno, M., Sun, X., Figueroa, J., Sherman, M. E., Garcia-Closas, M., Meltzer, P., et al.. (2014). Parity-related molecular signatures and breast cancer subtypes by estrogen receptor status. Breast Cancer Res, 16(4), R74. presented at the 2014.

Fagan-Solis, K. D., Pentecost, B. T., Gozgit, J. M., Bentley, B. A., Marconi, S. M., Otis, C. N., et al.. (2014). SKP2 overexpression is associated with increased serine 10 phosphorylation of p27 (pSer10p27) in triple-negative breast cancer. J Cell Physiol, 229(9), 1160-9. presented at the 2014 Sep.

Sturgeon, S. R., Arcaro, K. F., Johnson, M. A., Balasubramanian, R., Zorn, M., D Jerry, J., & Schneider, S. Smith. (2014). DNA methylation in paired breast epithelial and white blood cells from women undergoing reduction mammoplasty. Anticancer Res, 34(6), 2985-90. presented at the 2014 Jun.

Schneider, S. S., Aslebagh, R., Wetie, A. G. Ngounou, Sturgeon, S. R., Darie, C. C., & Arcaro, K. F.. (2014). Using breast milk to assess breast cancer risk: the role of mass spectrometry-based proteomics. Adv Exp Med Biol, 806, 399-408. presented at the 2014.

Yadava, N., Schneider, S. S., D Jerry, J., & Kim, C.. (2013). Impaired mitochondrial metabolism and mammary carcinogenesis. J Mammary Gland Biol Neoplasia, 18(1), 75-87. presented at the 2013 Mar.

Fagan-Solis, K. D., Schneider, S. Smith, Pentecost, B. T., Bentley, B. A., Otis, C. N., Gierthy, J. F., & Arcaro, K. F.. (2013). The ras homolog gene family, member A (RhoA) pathway mediates MMP-2 and MMP-9-independent invasive behavior in a triple-negative breast cancer cell line. J Cell Biochem. presented at the 2013 Jun.

Dunphy, K. A., Seo, J. - H., Kim, D. J., Roberts, A. L., Tao, L., Direnzo, J., et al.. (2013). Oncogenic transformation of mammary epithelial cells by transforming growth factor beta independent of mammary stem cell regulation. Cancer Cell Int, 13(1), 74. presented at the 2013 Jul 25.

Sun, X., Casbas-Hernandez, P., Bigelow, C., Makowski, L., D Jerry, J., Schneider, S. Smith, & Troester, M. A.. (2012). Normal breast tissue of obese women is enriched for macrophage markers and macrophage-associated gene expression. Breast Cancer Res Treat, 131(3), 1003-12. presented at the 2012 Feb.

Troester, M. A., Lee, M. Hee, Carter, M., Fan, C., Cowan, D. W., Perez, E. Roman, et al.. (2009). Activation of host wound responses in breast cancer microenvironment. Clin Cancer Res, 15(22), 7020-8. presented at the 2009 Nov 15.

Lu, S., Becker, K. A., Hagen, M. J., Yan, H., Roberts, A. L., Mathews, L. A., et al.. (2008). Transcriptional responses to estrogen and progesterone in mammary gland identify networks regulating p53 activity. Endocrinology, 149(10), 4809-20. presented at the 2008 Oct.

Lu, S., Becker, K. A., Hagen, M. J., Yan, H., Roberts, A. L., Mathews, L. A., et al.. (2008). Transcriptional responses to estrogen and progesterone in mammary gland identify networks regulating p53 activity. Endocrinology, 149(10), 4809-20. presented at the 2008 Oct.

Tu, Y., D Jerry, J., Pazik, B., & Schneider, S. Smith. (2005). Sensitivity to DNA damage is a common component of hormone-based strategies for protection of the mammary gland. Molecular cancer research : MCR, 3(8), 435-42. presented at the 2005 Aug.

Tu, Y., D Jerry, J., Pazik, B., & Schneider, S. Smith. (2005). Sensitivity to DNA damage is a common component of hormone-based strategies for protection of the mammary gland. Mol Cancer Res, 3(8), 435-42. presented at the 2005 Aug.

Becker, K. A., Lu, S., Dickinson, E. S., Dunphy, K. A., Mathews, L., Schneider, S. Smith, & D Jerry, J.. (2005). Estrogen and progesterone regulate radiation-induced p53 activity in mammary epithelium through TGF-beta-dependent pathways. Oncogene, 24(42), 6345-53. presented at the 2005 Sep 22.

Becker, K. A., Lu, S., Dickinson, E. S., Dunphy, K. A., Mathews, L., Schneider, S. Smith, & D Jerry, J.. (2005). Estrogen and progesterone regulate radiation-induced p53 activity in mammary epithelium through TGF-beta-dependent pathways. Oncogene, 24(42), 6345-53. presented at the 2005 Sep 22.

Osborne, B. A., Smith, S. W., McLaughlin, K. A., Grimm, L., Morgan, G., & Goldsby, R. A.. (1996). Genetic regulation of apoptosis in the mouse thymus. Advances in experimental medicine and biology, 406, 199-207. presented at the 1996.

Former Lab Personnel

Name
Bassa, Lotfi
Dudek, Maxine
Wong, Kaitlyn