| Title | Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells. |
| Publication Type | Journal Article |
| Year of Publication | 1998 |
| Authors | Cibelli, JB, Stice, SL, Golueke, PJ, Kane, JJ, Jerry, J, Blackwell, C, de León, FAPonce, Robl, JM |
| Journal | Nat Biotechnol |
| Volume | 16 |
| Issue | 7 |
| Pagination | 642-6 |
| Date Published | 1998 Jul |
| Keywords | Animals, Animals, Genetically Modified, beta-Galactosidase, Blastocyst, Cattle, Cell Differentiation, Cell Fusion, Chimera, Ectoderm, Embryo Transfer, Endoderm, Gene Transfer Techniques, Genetic Therapy, Mesoderm, Stem Cells |
| Abstract | We have developed a method, using nuclear transplantation, to produce transgenic embryonic stem (ES)-like cells from fetal bovine fibroblasts. These cells, when reintroduced into preimplantation embryos, differentiated into derivatives from the three embryonic germ layers, ectoderm, mesoderm, and endoderm, in 5-month-old animals. Six out of seven (86%) calves born were found to be chimeric for at least one tissue. These experiments demonstrate that somatic cells can be genetically modified and then de-differentiated by nuclear transfer into ES-like cells, opening the possibility of using them in differentiation studies and human cell therapy. |
| Alternate Journal | Nat. Biotechnol. |
Veterinary and Animal Sciences