Title | Protein-Antibody Conjugates (PACs): A Plug-and-Play Strategy for Covalent Conjugation and Targeted Intracellular Delivery of Pristine Proteins. |
Publication Type | Journal Article |
Year of Publication | 2021 |
Authors | Liu, B, Singh, K, Gong, S, Canakci, M, Osborne, BA, Thayumanavan, S |
Journal | Angew Chem Int Ed Engl |
Volume | 60 |
Issue | 23 |
Pagination | 12813-12818 |
Date Published | 2021 06 01 |
ISSN | 1521-3773 |
Keywords | Antibodies, Cell Line, Tumor, Fullerenes, Humans, Nanoparticles, Particle Size, Receptor, erbB-2 |
Abstract | We report here on protein-antibody conjugates (PACs) that are used for antibody-directed delivery of protein therapeutics to specific cells. PACs have the potential to judiciously combine the merits of two prolific therapeutic approaches-biologics and antibody-drug conjugates. We utilize spherical polymer brushes to construct PACs using the combination of two simple and efficient functionally orthogonal click chemistries. In addition to the synthesis and characterization of these nanoparticles, we demonstrate that PACs are indeed capable of specifically targeting cells based on the presence of target antigen on the cell surface to deliver proteins. The potentially broad adaptability of PACs opens up new opportunities for targeted biologics in therapeutics and sensing. |
DOI | 10.1002/anie.202103106 |
Alternate Journal | Angew Chem Int Ed Engl |
PubMed ID | 33768625 |
PubMed Central ID | PMC8762996 |
Grant List | R35 GM136395 / GM / NIGMS NIH HHS / United States GM-136395 / GM / NIGMS NIH HHS / United States |
Veterinary and Animal Sciences