Protein-Antibody Conjugates (PACs): A Plug-and-Play Strategy for Covalent Conjugation and Targeted Intracellular Delivery of Pristine Proteins.

TitleProtein-Antibody Conjugates (PACs): A Plug-and-Play Strategy for Covalent Conjugation and Targeted Intracellular Delivery of Pristine Proteins.
Publication TypeJournal Article
Year of Publication2021
AuthorsLiu, B, Singh, K, Gong, S, Canakci, M, Osborne, BA, Thayumanavan, S
JournalAngew Chem Int Ed Engl
Volume60
Issue23
Pagination12813-12818
Date Published2021 06 01
ISSN1521-3773
KeywordsAntibodies, Cell Line, Tumor, Fullerenes, Humans, Nanoparticles, Particle Size, Receptor, erbB-2
Abstract

We report here on protein-antibody conjugates (PACs) that are used for antibody-directed delivery of protein therapeutics to specific cells. PACs have the potential to judiciously combine the merits of two prolific therapeutic approaches-biologics and antibody-drug conjugates. We utilize spherical polymer brushes to construct PACs using the combination of two simple and efficient functionally orthogonal click chemistries. In addition to the synthesis and characterization of these nanoparticles, we demonstrate that PACs are indeed capable of specifically targeting cells based on the presence of target antigen on the cell surface to deliver proteins. The potentially broad adaptability of PACs opens up new opportunities for targeted biologics in therapeutics and sensing.

DOI10.1002/anie.202103106
Alternate JournalAngew Chem Int Ed Engl
PubMed ID33768625
PubMed Central IDPMC8762996
Grant ListR35 GM136395 / GM / NIGMS NIH HHS / United States
GM-136395 / GM / NIGMS NIH HHS / United States