Title | Molecular cloning of bovine chemokine receptors and expression by WC1+ gammadelta T cells. |
Publication Type | Journal Article |
Year of Publication | 2007 |
Authors | Blumerman, SL, Wang, F, Herzig, CTA, Baldwin, CL |
Journal | Developmental and comparative immunology |
Volume | 31 |
Issue | 1 |
Pagination | 87-102 |
Date Published | 2007 |
Abstract | Chemokine receptors mediate leukocyte migration into secondary lymphoid tissues and localization to peripheral inflammation sites. We describe full-length cDNA sequences of bovine chemokine receptors CCR5, CCR7, CXCR3 and CXCR5 and transcript expression by WC1(+)gammadelta T cells, a unique cell population with proinflammatory characteristics that comprises a large proportion of mononuclear cells in young ruminants. Bovine chemokine sequences were more similar to those of humans than were murine sequences to humans', ranging from 84% to 91%. Transcript analysis showed that antigen stimulation of WC1(+)gammadelta T cells induced IFN-gamma production and substantially increased CCR5 and CXCR3 expression when compared with freshly isolated (ex vivo) cells. CCR7 transcripts were minimally expressed in ex vivo and proliferating WC1(+)gammadelta T cells and CXCR5 expression was negligible. These results confirm the proinflammatory nature of WC1(+)gammadelta T cells is reflected by its chemokine receptor expression and suggest WC1(+)gammadelta T cells are unlikely to transit through secondary lymphoid tissues. |
Alternate Journal | Dev. Comp. Immunol. |
Veterinary and Animal Sciences