MCRS1 is essential for epiblast development during early mouse embryogenesis.

TitleMCRS1 is essential for epiblast development during early mouse embryogenesis.
Publication TypeJournal Article
Year of Publication2020
AuthorsCui, W, Cheong, A, Wang, Y, Tsuchida, Y, Liu, Y, Tremblay, KD, Mager, J
Date Published2020 01
KeywordsAnimals, Blastocyst Inner Cell Mass, Cell Differentiation, Cell Lineage, Embryo, Mammalian, Embryonic Development, Embryonic Stem Cells, Female, Gene Expression Regulation, Developmental, Germ Layers, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Mutation, RNA-Binding Proteins

Microspherule protein 1 (MCRS1, also known as MSP58) is an evolutionarily conserved protein that has been implicated in various biological processes. Although a variety of functions have been attributed to MCRS1 in vitro, mammalian MCRS1 has not been studied in vivo. Here we report that MCRS1 is essential during early murine development. Mcrs1 mutant embryos exhibit normal morphology at the blastocyst stage but cannot be recovered at gastrulation, suggesting an implantation failure. Outgrowth (OG) assays reveal that mutant blastocysts do not form a typical inner cell mass (ICM) colony, the source of embryonic stem cells (ESCs). Surprisingly, cell death and histone H4 acetylation analysis reveal that apoptosis and global H4 acetylation are normal in mutant blastocysts. However, analysis of lineage specification reveals that while the trophoblast and primitive endoderm are properly specified, the epiblast lineage is compromised and exhibits a severe reduction in cell number. In summary, our study demonstrates the indispensable role of MCRS1 in epiblast development during early mammalian embryogenesis.

Alternate JournalReproduction
PubMed ID31671403
PubMed Central IDPMC7431978
Grant ListR01 HD083311 / HD / NICHD NIH HHS / United States