%0 Journal Article %J Developmental and comparative immunology %D 2007 %T Molecular cloning of bovine chemokine receptors and expression by WC1+ gammadelta T cells. %A Blumerman, Seth L %A Wang, Fei %A Herzig, Carolyn T A %A Baldwin, Cynthia L %X Chemokine receptors mediate leukocyte migration into secondary lymphoid tissues and localization to peripheral inflammation sites. We describe full-length cDNA sequences of bovine chemokine receptors CCR5, CCR7, CXCR3 and CXCR5 and transcript expression by WC1(+)gammadelta T cells, a unique cell population with proinflammatory characteristics that comprises a large proportion of mononuclear cells in young ruminants. Bovine chemokine sequences were more similar to those of humans than were murine sequences to humans', ranging from 84% to 91%. Transcript analysis showed that antigen stimulation of WC1(+)gammadelta T cells induced IFN-gamma production and substantially increased CCR5 and CXCR3 expression when compared with freshly isolated (ex vivo) cells. CCR7 transcripts were minimally expressed in ex vivo and proliferating WC1(+)gammadelta T cells and CXCR5 expression was negligible. These results confirm the proinflammatory nature of WC1(+)gammadelta T cells is reflected by its chemokine receptor expression and suggest WC1(+)gammadelta T cells are unlikely to transit through secondary lymphoid tissues. %B Developmental and comparative immunology %V 31 %P 87-102 %8 2007 %G eng %N 1 %1 http://www.ncbi.nlm.nih.gov/pubmed/16762412?dopt=Abstract