%0 Journal Article %J Blood %D 2009 %T Notch signaling mediates G1/S cell-cycle progression in T cells via cyclin D3 and its dependent kinases. %A Joshi, Ila %A Minter, Lisa M %A Telfer, Janice %A Demarest, Renée M %A Capobianco, Anthony J %A Aster, Jon C %A Sicinski, Piotr %A Fauq, Abdul %A Golde, Todd E %A Osborne, Barbara A %X Notch signaling plays a role in normal lymphocyte development and function. Activating Notch1-mutations, leading to aberrant downstream signaling, have been identified in human T-cell acute lymphoblastic leukemia (T-ALL). While this highlights the contribution of Notch signaling to T-ALL pathogenesis, the mechanisms by which Notch regulates proliferation and survival in normal and leukemic T cells are not fully understood. Our findings identify a role for Notch signaling in G(1)-S progression of cell cycle in T cells. Here we show that expression of the G(1) proteins, cyclin D3, CDK4, and CDK6, is Notch-dependent both in vitro and in vivo, and we outline a possible mechanism for the regulated expression of cyclin D3 in activated T cells via CSL (CBF-1, mammals; suppressor of hairless, Drosophila melanogaster; Lag-1, Caenorhabditis elegans), as well as a noncanonical Notch signaling pathway. While cyclin D3 expression contributes to cell-cycle progression in Notch-dependent human T-ALL cell lines, ectopic expression of CDK4 or CDK6 together with cyclin D3 shows partial rescue from gamma-secretase inhibitor (GSI)-induced G(1) arrest in these cell lines. Importantly, cyclin D3 and CDK4 are highly overexpressed in Notch-dependent T-cell lymphomas, justifying the combined use of cell-cycle inhibitors and GSI in treating human T-cell malignancies. %B Blood %V 113 %P 1689-98 %8 2009 Feb 19 %G eng %N 8 %1 http://www.ncbi.nlm.nih.gov/pubmed/19001083?dopt=Abstract