TY - JOUR T1 - Cloned transgenic calves produced from nonquiescent fetal fibroblasts. JF - Science Y1 - 1998 A1 - Cibelli, J B A1 - Stice, S L A1 - Golueke, P J A1 - Kane, J J A1 - Jerry, J A1 - Blackwell, C A1 - Ponce de León, F A A1 - Robl, J M KW - Animals KW - Animals, Genetically Modified KW - Blastocyst KW - Cattle KW - Cell Aging KW - Cell Division KW - Cell Nucleus KW - Cells, Cultured KW - Clone Cells KW - Cloning, Organism KW - Embryo Transfer KW - Female KW - Fetus KW - Fibroblasts KW - G1 Phase KW - Male KW - Nuclear Transfer Techniques KW - Oocytes KW - Transfection KW - Transgenes AB -

An efficient system for genetic modification and large-scale cloning of cattle is of importance for agriculture, biotechnology, and human medicine. Here, actively dividing fetal fibroblasts were genetically modified with a marker gene, a clonal line was selected, and the cells were fused to enucleated mature oocytes. Out of 28 embryos transferred to 11 recipient cows, three healthy, identical, transgenic calves were generated. Furthermore, the life-span of near senescent fibroblasts could be extended by nuclear transfer, as indicated by population doublings in fibroblast lines derived from a 40-day-old fetal clone. With the ability to extend the life-span of these primary cultured cells, this system would be useful for inducing complex genetic modifications in cattle.

VL - 280 IS - 5367 U1 -

http://www.ncbi.nlm.nih.gov/pubmed/9596577?dopt=Abstract

ER -