02885nas a2200433 4500008004100000022001400041245017200055210006900227260000900296300001300305490000700318520145000325653002401775653001201799653003401811653002301845653003501868653002801903653002401931653002101955653001101976653002001987653001202007653000902019653002802028653002902056653001402085653002402099100001602123700001902139700002202158700002102180700001802201700001902219700001902238700001702257700002602274856015102300 2015 eng d a1932-620300aA glycine insertion in the estrogen-related receptor (ERR) is associated with enhanced expression of three cytochrome P450 genes in transgenic Drosophila melanogaster.0 aglycine insertion in the estrogenrelated receptor ERR is associa c2015 ae01187790 v103 a
Insecticide-resistant Drosophila melanogaster strains represent a resource for the discovery of the underlying molecular mechanisms of cytochrome P450 constitutive over-expression, even if some of these P450s are not directly involved in the resistance phenotype. For example, in select 4,4'-dichlorodiphenyltrichloroethane (DDT) resistant strains the glucocorticoid receptor-like (GR-like) potential transcription factor binding motifs (TFBMs) have previously been shown to be associated with constitutively differentially-expressed cytochrome P450s, Cyp12d1, Cyp6g2 and Cyp9c1. However, insects are not known to have glucocorticoids. The only ortholog to the mammalian glucocorticoid receptor (GR) in D. melanogaster is an estrogen-related receptor (ERR) gene, which has two predicted alternative splice isoforms (ERRa and ERRb). Sequencing of ERRa and ERRb in select DDT susceptible and resistant D. melanogaster strains has revealed a glycine (G) codon insertion which was only observed in the ligand binding domain of ERR from the resistant strains tested (ERR-G). Transgenic flies, expressing the ERRa-G allele, constitutively over-expressed Cyp12d1, Cyp6g2 and Cyp9c1. Only Cyp12d1 and Cyp6g2 were over-expressed in the ERRb-G transgenic flies. Phylogenetic studies show that the G-insertion appeared to be located in a less conserved domain in ERR and this insertion is found in multiple species across the Sophophora subgenera.
10aAmino Acid Sequence10aAnimals10aAnimals, Genetically Modified10aConserved Sequence10aCytochrome P-450 Enzyme System10aDrosophila melanogaster10aDrosophila Proteins10aEnzyme Induction10aFemale10aGene Expression10aGlycine10aMale10aMolecular Sequence Data10aMutagenesis, Insertional10aPhylogeny10aReceptors, Estrogen1 aSun, Weilin1 aValero, Carmen1 aSeong, Keon, Mook1 aSteele, Laura, D1 aHuang, I-Ting1 aLee, Chien-Hui1 aClark, John, M1 aQiu, Xinghui1 aPittendrigh, Barry, R uhttps://www.umass.edu/veterinary-animal-sciences/research-faculty/publications/glycine-insertion-estrogen-related-receptor-err-associated-enhanced