02001nas a2200157   4500008004100000245012400041210006900165260001300234300001100247490000700258520135200265100002501617700002301642700002401665856015401689       2006                        eng d00aIdentification of three new bovine T-cell receptor delta variable gene subgroups expressed by peripheral blood T cells.0 aIdentification of three new bovine Tcell receptor delta variable  c2006 Sep  a746-570 v583 aTo understand the biology of gammadelta T cells in ruminants, it is necessary to have a comprehensive picture of gammadelta T-cell receptor gene diversity and expression. In this study, three new subgroups of bovine T-cell receptor delta (TRD) variable genes were identified by RT-PCR and sequencing and homology with TRDV genes from other mammals determined. Previously unidentified TRDV subgroup genes described in this study include the bovine homologues of ovine TRDV2, TRDV3, and TRDV4 which were named accordingly. TRDV2 subgroup has two genes (TRDV2-1 and TRDV2-2) while we found the previously identified TRDV1 has at least eight genes corresponding to separate genomic sequences. Nucleotide and amino acid sequences for particular gene subgroups between cattle and sheep were more than 87% identical but identities among TRDV subgroups within a species were much less, with bovine TRDV4 having <45% identity to the other three bovine TRDV gene subgroups. Analysis of circulating bovine gammadelta T cells revealed that genes from all four TRDV subgroups were expressed in combination with TRDJ1, TRDJ3, and TRDC, although TRDV4 was the least represented, and all displayed a variety of CDR3 junctional lengths. Finally, some genes within the TRDV1, TRDV2, and TRDV3 subgroups recombined with TRAV incorporating TRAJs, suggesting dual use.1 aHerzig, Carolyn, T A1 aBlumerman, Seth, L1 aBaldwin, Cynthia, L  uhttps://www.umass.edu/veterinary-animal-sciences/research-faculty/publications/identification-of-three-new-bovine-t-cell-receptor-delta-variable-gene