02070nas a2200241   4500008004100000245012400041210006900165260001600234300001100250490000800261520119900269100001501468700002101483700001401504700002501518700002001543700002601563700002501589700002201614700001801636700002301654856015101677       2008                        eng d00aInositol 1,4,5-trisphosphate receptor 1, a widespread Ca2+ channel, is a novel substrate of polo-like kinase 1 in eggs.0 aInositol 145trisphosphate receptor 1 a widespread Ca2 channel is  c2008 Aug 15  a402-130 v3203 a<p>To initiate embryo development, the sperm induces in the egg release of intracellular calcium ([Ca2+](i)). During oocyte maturation, the inositol 1,4,5-trisphosphate receptor (IP(3)R1), the channel implicated, undergoes modifications that enhance its function. We found that IP(3)R1 becomes phosphorylated during maturation at an MPM-2 epitope and that this persists until the fertilization-associated [Ca2+](i) responses cease. We also reported that maturation without ERK activity diminishes IP(3)R1 MPM-2 reactivity and [Ca2+](i) responses. Here, we show that IP(3)R1 is a novel target for Polo-like kinase1 (Plk1), a conserved M-phase kinase, which phosphorylates it at an MPM-2 epitope. Plk1 and IP(3)R1 interact in an M-phase preferential manner, and they exhibit close co-localization in the spindle/spindle poles area. This co-localization is reduced in the absence of ERK activity, as the ERK pathway regulates spindle organization and IP(3)R1 cortical re-distribution. We propose that IP(3)R1 phosphorylation by Plk1, and possibly by other M-phase kinases, underlies the delivery of spatially and temporally regulated [Ca2+](i) signals during meiosis/mitosis and cytokinesis.</p>
1 aIto, Junya1 aYoon, Sook-Young1 aLee, Bora1 aVanderheyden, Veerle1 aVermassen, Elke1 aWojcikiewicz, Richard1 aAlfandari, Dominique1 aDe Smedt, Humbert1 aParys, Jan, B1 aFissore, Rafael, A  uhttps://www.umass.edu/veterinary-animal-sciences/research-faculty/publications/inositol-145-trisphosphate-receptor-1-a-widespread-ca2-channel-is-a