@article {11908,
	title = {Regulation of the composition of the extracellular matrix by low density lipoprotein receptor-related protein-1: activities based on regulation of mRNA expression.},
	journal = {The Journal of biological chemistry},
	volume = {281},
	year = {2006},
	month = {2006 Mar 17},
	pages = {7332-40},
	abstract = {Low density lipoprotein receptor-related protein-1 (LRP-1) is a catabolic receptor for extracellular matrix (ECM) structural proteins and for proteins that bind to ECM. LRP-1 also is implicated in integrin maturation. In this study, we applied a proteomics strategy to identify novel proteins involved in ECM modeling that are regulated by LRP-1. We show that LRP-1 deficiency in murine embryonic fibroblasts (MEFs) is associated with increased levels of type III collagen and pigment epithelium-derived factor, which accumulate in the substratum surrounding cells. The collagen receptor, uPAR-AP/Endo-180, is also increased in LRP-1-deficient MEFs. Human LRP-1 reversed the changes in protein expression associated with LRP-1 deficiency; however, the endocytic activity of LRP-1 was not involved. Instead, regulation occurred at the mRNA level. Inhibition of c-Jun amino-terminal kinase (JNK) blocked type III collagen expression in LRP-1-deficient MEFs, suggesting regulation of JNK activity as a mechanism by which LRP-1 controls mRNA expression. The ability of LRP-1 to regulate expression of the factors identified here suggests a role for LRP-1 in determining blood vessel structure and in angiogenesis.},
	keywords = {Animals, Biotin, Cell Line, Cell Membrane, Cloning, Molecular, Collagen, Culture Media, Conditioned, Electrophoresis, Gel, Two-Dimensional, Electrophoresis, Polyacrylamide Gel, Endocytosis, Extracellular Matrix, Fibrinogen, Gene Expression Regulation, Humans, LDL-Receptor Related Proteins, Low Density Lipoprotein Receptor-Related Protein-1, Mass Spectrometry, Mice, Microscopy, Fluorescence, Neovascularization, Pathologic, Phosphorylation, Proteomics, Receptors, LDL, Reverse Transcriptase Polymerase Chain Reaction, RNA, Messenger, Signal Transduction, Surface Properties, Tumor Suppressor Proteins},
	author = {Gaultier, Alban and Salicioni, Ana Maria and Arandjelovic, Sanja and Gonias, Steven L}
}
