| Title | Detection and differentiation of normal, cancerous, and metastatic cells using nanoparticle-polymer sensor arrays. |
| Publication Type | Journal Article |
| Year of Publication | 2009 |
| Authors | Bajaj, A, Miranda, OR, Kim, I-B, Phillips, RL, D Jerry, J, Bunz, UHF, Rotello, VM |
| Journal | Proceedings of the National Academy of Sciences of the United States of America |
| Volume | 106 |
| Issue | 27 |
| Pagination | 10912-6 |
| Date Published | 2009 Jul 7 |
| Abstract | Rapid and effective differentiation between normal and cancer cells is an important challenge for the diagnosis and treatment of tumors. Here, we describe an array-based system for identification of normal and cancer cells based on a "chemical nose/tongue" approach that exploits subtle changes in the physicochemical nature of different cell surfaces. Their differential interactions with functionalized nanoparticles are transduced through displacement of a multivalent polymer fluorophore that is quenched when bound to the particle and fluorescent after release. Using this sensing strategy we can rapidly (minutes/seconds) and effectively distinguish (i) different cell types; (ii) normal, cancerous and metastatic human breast cells; and (iii) isogenic normal, cancerous and metastatic murine epithelial cell lines. |
| Alternate Journal | Proc. Natl. Acad. Sci. U.S.A. |
Veterinary and Animal Sciences