Deltamethrin increases the fat accumulation in 3T3-L1 adipocytes and Caenorhabditis elegans.

TitleDeltamethrin increases the fat accumulation in 3T3-L1 adipocytes and Caenorhabditis elegans.
Publication TypeJournal Article
Year of Publication2017
AuthorsShen, P, Hsieh, T-H, Yue, Y, Sun, Q, Clark, JM, Park, Y
JournalFood Chem Toxicol
Date Published2017 Mar
Keywords3T3-L1 Cells, Acetyl-CoA Carboxylase, Adipocytes, Adipogenesis, AMP-Activated Protein Kinases, Animals, Caenorhabditis elegans, CCAAT-Enhancer-Binding Proteins, Insecticides, Locomotion, Mice, Nitriles, Phosphorylation, Pyrethrins, Triglycerides

Research has shown that permethrin, a Type-I pyrethroid, increases triglyceride (fat) accumulation in adipocytes. Little is known, however, about any similar effect of deltamethrin, a Type-II pyrethroid, which produces a distinct syndrome of poisoning in mammals compared with permethrin. This study was therefore aimed to explore the role of deltamethrin on fat accumulation in 3T3-L1 adipocytes and Caenorhabditis elegans. Deltamethrin (10 μM) significantly increased the fat accumulation in 3T3-L1 adipocytes and wild type C. elegans compared to respective controls. Deltamethrin decreased the ratio of phosphorylated AMP-activated kinase (pAMPKα) over AMPKα and phosphorylated acetyl-CoA carboxylase (ACC) over ACC, while it increased expression of CCAAT/enhancer-binding protein (C/EBPα) and peroxisome proliferator-activated receptor-γ (PPARγ) in 3T3-L1 adipocytes. Similarly, deltamethrin potentiated fat accumulation in C. elegans without affecting growth or pharyngeal pumping rate. Moreover, deltamethrin significantly reduced the total progeny number and locomotive activities in C. elegans in a dose-dependent manner. Deltamethrin increased fat accumulation via aak-2 (an ortholog of AMPKα) and nhr-49 (a homolog of peroxisome proliferator-activated receptor-α and also downstream target of aak-2) mediated mechanisms. The current work is the first report of the effects of deltamethrin on increased fat storage by 3T3- L1 adipocytes and C. elegans via aak-2 (AMPKα ortholog)-mediated mechanism.

Alternate JournalFood Chem Toxicol
PubMed ID28119079