Cancer Cell Discrimination Using Host-Guest "Doubled" Arrays.

TitleCancer Cell Discrimination Using Host-Guest "Doubled" Arrays.
Publication TypeJournal Article
Year of Publication2017
AuthorsLe, NDB, Tonga, GYesilbag, Mout, R, Kim, S-T, Wille, ME, Rana, S, Dunphy, KA, D Jerry, J, Yazdani, M, Ramanathan, R, Rotello, CM, Rotello, VM
JournalJ Am Chem Soc
Date Published2017 06 14
KeywordsBinding Sites, Biosensing Techniques, Bridged-Ring Compounds, Cell Line, Tumor, Gold, Humans, Imidazoles, Luminescent Proteins, Metal Nanoparticles, Molecular Structure, Nanotechnology, Neoplasms

We report a nanosensor that uses cell lysates to rapidly profile the tumorigenicity of cancer cells. This sensing platform uses host-guest interactions between cucurbit[7]uril and the cationic headgroup of a gold nanoparticle to non-covalently modify the binding of three fluorescent proteins of a multi-channel sensor in situ. This approach doubles the number of output channels to six, providing single-well identification of cell lysates with 100% accuracy. Significantly, this classification could be extended beyond the training set, determining the invasiveness of novel cell lines. The unique fingerprint of these cell lysates required minimal sample quantity (200 ng, ∼1000 cells), making the methodology compatible with microbiopsy technology.

Alternate JournalJ Am Chem Soc
PubMed ID28535040
PubMed Central IDPMC5848078
Grant ListR01 GM077173 / GM / NIGMS NIH HHS / United States