Students will receive practical experience in basic and advanced cell and molecular biology techniques used in biotechnology and research laboratories. The course first focuses on cell culture techniques, including seeding, maintenance, and cryopreservation. Subsequently, the differences between molecular analysis of transcription and translation will be addressed (RNA/cDNA, protein). For these analyses, cell cultures in their native form will be compared with bleomycin induced DNA damaged cell cultures. Overall, this course establishes a general understanding of cell growth physiology. In the later part, in situ detection of Ki-67 while preserving cytoarchitecture as well as FACS analysis complete the picture of cell growth.
NOTE: This course will not be offered in Spring 2024.