The paper entitled, "On the Interfacial Assembly of Anisotropic Amphiphilic Janus particles was recently published by Alex McGlasson*, Eva Morgenthaler*, Laura C. Bradley* and Thomas P. Russell* in Advanced Functional Materials. In this work, we study the interfacial assembly mechanism of anisotropic amphiphilic Janus particles. This article provides new insights into controlling the kinetics and equilibrium configuration of Janus particles at interfaces.