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Annette Wysocki

Annette Wysocki, PhD, RN, FAAN

Associate Dean for Research and Professor
College of Nursing
651 North Pleasant St.
Amherst, MA 01003-9299
413-545-5087 Telephone
413-577-2550 Facsimile
Skinner 138



Dr. Wysocki served as the first Scientific Director of the Division of Intramural Research at the National Institute of Nursing Research (NINR), National Institutes of Health (NIH).  She received her Ph.D. from the University of Texas at Austin and her baccalaureate and master’s degrees from East Carolina University.  She completed two postdoctoral fellowships at UT-Southwestern in Dallas, Texas and Weill-Cornell Medical College in New York City.

Her program of research is focused on understanding the biological mechanisms responsible for delayed healing in chronic wounds.  She combines the use of clinical materials, such as wound fluid and tissue, with the powerful tools of basic science to discover the mechanisms underlying the pathophysiology of delayed healing.  She discovered the degradation of fibronectin in chronic wounds, the activation and over-expression of matrix metalloproteinases (MMP-2; MMP-9) and serine proteases in chronic wound fluid; and identified bacteria capable of secreting proteases that can degrade extracellular matrix substrates important for healing.

Ongoing efforts are directed at studying how cells respond to different environmental conditions that may affect cell migration using an in vitro wound model and dermal equivalent.  Collaborative efforts have been directed at projects on keloids, pressure ulcers, colonizing and infecting bacteria, bacterial proteases, and topical antiseptic agents.  More recently her laboratory has also pursued studies of POSS-based nanomaterials to develop cartilage/synovial fluid substitutes.  And she has taught and presented on bioethics.

She is recognized as an expert in wound healing and has presented her research findings nationally and internationally and consults with pharmaceutical, cosmetic, and wound healing companies.  While at the NINR/NIH, she created, developed and implemented the Summer Genetics Institute; was appointed to serve on many NIH committees; and participated in several key NIH initiatives.   She has served on the Board of Directors of the Wound Healing Society and as President and Board Member of the Wound Healing Society Foundation. She has held previous academic and research appointments at New York University Medical Center as an Assistant Professor in the Department of Dermatology and Director of Nursing Research; and at the University of Mississippi Medical Center as a tenured Professor in the School of Nursing and School of Medicine, Department of Surgery.



BSN                East Carolina University (1978), Nursing 
MSN               East Carolina University (1980), Nursing
PhD                University of Texas at Austin (1986), Nursing
Postdoc          UT-Southwestern (1986-1989), Cell Biology
Postdoc          Weill Cornell Medical College (1989-1991), Cell Biology
Certificate       Georgetown University (2010), Kennedy Institute of Ethics, Bioethics
Certificate       Millsaps College (2011), Else School of Management, Business

Research Interests

Wound healing
Degradation of extracellular matrix
Cell adhesion and migration
Tissue engineering and biomaterials
Skin biology
Human research ethics
Research policy


N710: Quantitative Research Methods
Institute for Social Science Research


9/21/10 – 3/25/11  Co-Investigator: “Hemostatic Agent Development.” Office of Naval Research, Contract # N00014-10-M-0481. Subcontract         Award from Hybrid Plastics, Total Direct Cost: $23,750.
1/1/10 – 12/31/10  Principal Investigator:  “Engineering and Testing of a Bionanohybrid Cartilage Using POSS and Type II Collagen.”  National Science Foundation EPSCoR Seed Grant, Total Direct Cost:  $38,625.
1/10/06 – 8/30/06  Principal Investigator:  “Development of an In Vitro Wound Model.”  University of Mississippi Medical Center, School of Nursing, Seed Grant, Total Direct Cost: $3,000.
10/1/04-9/30/05  Principal Investigator: “The Chronic Wound Microenvironment in Aging.”  University of Mississippi Intramural Research Support, Total Direct Cost: $7,500.
10/2000 – 7/2002  Director/Principal Investigator:  Wound Healing Program, Intramural Research Program, National Institutes of Health, National Institute of Dental and Craniofacial Research, Bethesda, MD.  Intramural funding.
9/1997-9/2000  Laboratory Chief/Principal Investigator:  Wound Healing Laboratory, Intramural Research Program, National Institutes of Health, National Institute of Nursing Research, Bethesda, MD.  Intramural funding.
8/1/95-7/31/97  Principal Investigator:  "Fibronectin mRNA in the Chronic Wound Environment," National Institutes of Health, National Institute of Nursing Research, Total Direct Cost:  $38,513 (RO3 NR03809).
8/1/94-7/31/99  Principal Investigator:  "The Chronic Wound Environment in Spinal Cord Injury," National Institutes of Health, National Institute of Nursing Research, Total Direct Cost:  $590,174 (RO1 NR03898).
3/1/93-2/28/98  Principal Investigator:  "Chronic Wound Fluids:  Effect on Matrix and Cells," National Institutes of Health, National Institute of Nursing Research, Total Direct Cost: $346,468 (R29 NR03212).
7/01/88-6/30/91  Postdoctoral Research Fellow:  "Analysis of Cutaneous Wound Fluids."  Sponsor:  Fred Grinnell and Lisa Staiano-Coico.  National Institutes of Health, National Institute of Nursing Research, Individual National Research Service Award,  Fellowship Support:  $84,746 (F32 NR06263).
10/1/84-9/30/85  Principal Investigator:  "Effect of Intermittent Noise Exposure on Wound Healing in Albino Rats," American Nurses' Foundation Competitive Grant, Total Direct Cost:  $2,396.37.



Wysocki, A. B., Bhalla, S., Tierno, Jr., P. M., Stevens-Riley, M., & Propst, R-C.  (2013).  Proteolytic activity by multiple bacterial species isolated from chronic venous leg ulcers degrades matrix substrates.  Biological Research in Nursing, 15, 407-415. DOI: 10.1177/1099800412464683 Article was featured on the www. site on 2/25/13 and was assigned to the following specialties: Internal Medicine, Nursing, Surgery, Infectious Disease, Emergency Medicine, Medical Student, Nurse Practitioner, Physician Assistant.

Tuan, T.-L., Wysocki, A., Grinnell, F. (2013).  Contemporary challenges facing junior scientists in biomedical research.  Wound Healing Society Newsletter, 4(1): 3-5.

Lee, Y-S., Wysocki, A., Warburton, D., Tuan, T-L.  (2012). Wound healing in development. Birth Defects Research Part C: Embryo Today: Reviews, 96: 213–222. doi: 10.1002/bdrc.21017

Wysocki, A. B., Tran, A., Janorkar, A. V.  (2012).  Cell migration and proliferation in bionanohybrids composed of Type II collagen and POSS amphiphiles.  NanoLIFE, 2, 1241001-1 -10.   DOI: 10.1142/S1793984412410012.

Vick, L. R.,  Propst, R. C.,  Bozeman, R.,  & Wysocki, A. B.. (2009).  The effect of Dakin’s solution on components of a dermal equivalent.  Journal of Surgical Research, 155, 54-64.  (4 citations)

Frank, D. N., Wysocki, A. B., Specht-Glick,  D. D., Rooney,  A., Feldman, R. A., St. Amand, A., Pace, N. R.,  & Trent,  J. (2009). Microbial diversity in chronic open wounds.  Wound Repair and Regeneration, 17, 163-172.  (16 citations)

*Schultz, G.S.,  & Wysocki, A. (2009).  Interactions between extracellular matrix and growth factors in wound healing.  Wound Repair and Regeneration, 17, 153-162.  (98 citations)

Tuan, T.-L., Hwu, P., Ho, W., Yiu, P., Chang., R., Wysocki, A., & Benya, P. (2008). Adenoviral overexpression and small interfering RNA suppression demonstrate that plasminogen activator inhibitor-1 produces elevated collagen accumulation in normal and keloid fibroblasts.  American Journal of Pathology, 173, 1311-1325.  (5 citations)

Wysocki, A. B., Dorsett-Martin, W., A.  (2008).  Skin grafts.  OR Nurse 2008, 2, 30-38.

Malinda, K. M., Wysocki, A. B.,  Koblinski, J., Kleinman, H. K.,  & Ponce, M. L. (2008).  Angiogenic laminin peptides stimulate wound healing.  International Journal of Biochemistry and Cell Biology, 40, 2771-2780. (5 citations)

Dorsett-Martin, W. A., Persons, B. L., Wysocki, A. B.,  & Lineaweaver, W. C.,  (2008).  New topical agents for treatment of partial-thickness burns in children:  A review of published outcome studies.  Wounds, 20, 292-298.

Dudas, M , Wysocki, A. B., Gelpi, B.,  & Tuan, T.-L. (2008).  Memory encoded throughout our bodies:  Molecular and cellular basis of tissue regeneration.  PediatricResearch, 63, 502-512. (6 citations)

Schultz, G. S., Ladwig,  G.,  & Wysocki, A. B.  (2005).  Extracellular matrix:  Review of its roles in acute and chronic wounds. World  Wide Wounds.  Retrieved from

*Wysocki, A. B.,  & Bookbinder M. (2005).  Implementing clinical practice changes:  A practical approach.  Home Health Care Management & Practice, 17, 164-174.  (#1 of the 50 Most-Frequently Read/Downloaded Articles in October 2008).

Hott, A. M., Huether, C. A., McInerney, J. D., Christianson,  C., Fowler,  R., Bender,  H., Jenkins,  J., Wysocki,  A., Markle,  G.,  & Karp,  R. (2002) Genetics content in introductory biology courses for non-science majors: Theory and practice.  Bioscience, 52, 1024-1035.  (6 citations)

*Wysocki, A. B. (2002).  Evaluating and managing open skin wounds:  Colonization versus infection.  AAACN Clinical Issues:  Advanced Practice in Acute and Critical Care:  Emerging Infections, 13, 382-397.  (37 citations)

Wysocki,  A. B. (2001)  Elaboration D’Un Protocole De Recherche Clinique (Developing a Clinical Research Protocol).  Journal des Plaies et Cicatrisations, 6, 6-11.         

*Wysocki, A. B., Kusakabe, A. O., Chang, S.,  & Tuan, T.-L.  (1999).  Temporal expression of urokinase plasminogen activator, plasminogen activator inhibitor and gelatinase-B in chronic chronic wound fluid switches from a chronic to acute wound profile with progression to healing.  Wound Repair and Regeneration, 7, 154-165. (60 citations)

Wysocki, A. B. (1999).  Skin anatomy, physiology, and pathophysiology.  Nursing Clinics of North America, 34(4), 777-797.  (13 citations)

Wysocki, A. B.  (1998).  Launching your research career through postdoctoral training opportunities.  Nursing Research, 47(3), 127-128.

Gray, M.,  & Wysocki, A. B.  (1997).  Bridging the gap from bench to bedside in wound care.  Journal of WOCN, 24(2), 62-65.

Wysocki, A. B.  (1997).  Wound measurement - reply.  International Journal of Dermatology, 36(5), 399.

*Wysocki, A. B.  (1996).  Wound fluids and the pathogenesis of chronic wounds.  Journal of WOCN, 23(6), 283-290.  (38 citations)

*Chang J. C., Wysocki, A., Tchou-Wong, K-M., Moskowitz, N., Zhang, Y., & Rom W. N.  (1996).  Effect of Mycobacterium tuberculosis  and its components on macrophage release of matrix metalloproteinases.  Thorax, 51(3), 306-311. (87 citations)

Wysocki, A. B.  (1996).  The effect of intermittent noise exposure on excisional wound healing.  Advances in Wound Care, 9(1), 35-39.  (19 citations)

Wysocki, A. B. (1996).  Wound measurement:  A review.  International Journal of Dermatology, 35(2), 82-91.  (18 citations)

Wysocki, A. B. (1995).  A review of the skin and its appendages.  Advances in WoundCare, 8(2), 53-70.  [published erratum appears in Advances in Wound Care, 8(4), 8.]  (4 citations)

**Wysocki, A. B., Staiano-Coico, L., & Grinnell, F. (1993).  Wound fluid from chronic leg ulcers contains elevated levels of metalloproteinases MMP-2 and MMP-9.  Journal of Investigative Dermatology, 101(1), 64-68. (*350 citations; Ranked #11 of the 50 most cited articles published in the Journal of Investigative Dermatology for the past 25 years)

Wysocki, A. B.  (1992).  Fibronectin in acute and chronic wounds.  Journal of ET Nursing, 19(5), 166-170. (6 citations)

**Grinnell, F., Ho, C-H., & Wysocki, A. B.  (1992).  Degradation of fibronectin and vitronectin in chronic wound fluid:  Analysis by cell blotting, immunoblotting, and cell adhesion assays.  Journal of Investigative Dermatology, 98(4), 410-416. (*149 citations)

*Wysocki, A. B. & Grinnell, F. (1990).  Fibronectin profiles in normal and chronic wound fluid.  Laboratory Investigation, 63(6), 825-831. (78 citations)

Thomas, A. C. & Wysocki, A. B.  (1990)  The healing wound:  A comparison of three clinically useful methods of measurement.  Decubitus, 3(1), 18-20, 24-25.  (7 citations)

Wysocki, A. B. (1989).  Surgical wound healing:  A review for perioperative nurses.  AORN Journal, 49(2), 502-518.  (2 citations)

Wysocki, A., Baxter,  C. R., Bergstresser, P.  R., Grinnell, F., Horowitz,  M. S., & Horowitz, B. (1988).  Topical fibronectin therapy for treatment of a patient with chronic stasis ulcers.  Archives of Dermatology, 124(2), 175-177.  (15 citations)

Kim, N. H., Wysocki, A. B., Bovik, A. C. & Diller, K. R. (1987).  A microcomputer based vision system for area measurement.  Computers in Biology and Medicine, 17(3), 173-183.  (10 citations)

Wysocki, A. B. (1983).  Basic versus applied research:  Intrinsic and extrinsic considerations.  Western Journal of Nursing Research, 5(3), 217-224.

Wysocki, A.  (1980).  Characteristics of nursing role models.  Carolina Journal of Educational Research, 1, 24-29.



American Academy of Nursing, Fellow (FAAN), November 2, 2002
American Nurses' Foundation Scholar, 1984-1985
Annual Research Award, Theta Beta Chapter, Sigma Theta Tau, 2004
Award of Excellence, The Wound Healing Center, Medical College of Virginia, Richmond, VA, 1998
Distinguished Lecturer, University of South Florida, School of Nursing, May 2, 2007
Distinguished Service Award, Wound Healing Society, 2002
East Carolina University, Outstanding Alumni Award, 1999
East Carolina University, 100 Incredible ECU Women, Centennial Celebration, 2007
East Carolina University, Hall of Fame, College of Nursing, 2011
Fellow, University of Texas at Austin
Friends of the National Institute of Nursing Research, Certificate of Merit
Harvard Macy Scholar, Harvard Macy Institute, Program for Leaders in Medical Education, 2001
Jan Magee-Evers Faculty Fellowship Award, School of Nursing. U. of  Mississippi Medical Center, 2004

Professional Associations/Affiliations

American Association for the Advancement of Science (AAAS)
American Heart Association, Council on Nutrition, Physical Activity, and Metabolism, Charter Member
American Nurses Association (ANA)
American Society for Cell Biology (ASCB)
Eastern Nursing Research Society (ENRS)
Gamma Beta Phi (Academic Honor Society)
New York Academy of Sciences (NYAS)
Phi Kappa Phi (Academic Honor Society)
Southern Nursing Research Society (SNRS)
Wound Healing Society (WHS)
Sigma Theta Tau (Academic Honor Society)