Flow Cytometry

Flow Cytometry


Core Facilities Seminar

David Luther, (Rotello group) Department of Chemistry–Quantifying cytosolic protein delivery through imaging flow cytometry

Replay on YouTube

Designed to assist members of the research community at UMass and on the other Five College campuses to conduct research using flow cytometry and live animal imaging technologies. The equipment and services provided by the facility are unmatched on campus and in the five college area with the nearest facility at UMass Medical School in Worcester, more than one hour’s drive from campus.

  • Amnis ImageStream Mark II Imaging Flow Cytometer

    Combines the speed, sensitivity, and phenotyping abilities of flow cytometry with the detailed imagery and functional insights of microscopy.


  • BD DUAL LSRFortessa

    Analysis Capabilities for TWO USERS Simultaneously (the third such instrument in the country)

    • Left side – 3 Excitation Lasers: 405nm, 488nm, and 640nm, High Throughput Sampler 15 color analysis capabilities.
    • Right side – 5 Excitation Lasers: 355nm (UV), 405nm, 488nm, 561nm, and 640nm 18 color analysis capabilities.
    • (Optical Configuration Details) Manufacturers Link

  • BD FACSAria Fusion

    Streamlines sorting and analysis with its fixed alignment flow cell, fixed optical architecture and software-controlled setup and operation.

    • Index sorting capabilities
    • 4 Excitation Lasers-488, 640, 561 and 405-nm (no UV)
    • Class II Type A2 biosafety cabinet

  • Cytek Aurora

    Full spectrum technology allows up to:

    • 40 colors
    • Five lasers
    • 64 fluorescence channels
    • State-of-the-art optics and low-noise electronics
    • View multiple panels simultaneously.

Service Line Campus Users Other Academic Users Industry
  Hourly Rates
LSR Fortessa-with Operator $80 $95 $130
LSR Fortessa-without Operator $48 $65 $95
Amnis ImageStream-with Operator $80 $95 $130
Amnis ImageStream-without Operator $48 $65 $95
Aria - Sorting $115 $150 $235
Cytek-with Operator $80 $95 $130
Cytek-without Operator $48 $65 $95
Sony-BSL3 Sorter $160 N/A N/A
Director Consultation $105 $150 $250
Rates will be adjusted every fiscal year, so they are subject to change, contact facility to verify current fees.  
FY25 Specialized Service Center Approved Fees  

Contact the facility manager for the training schedule. Training on the flow analysis equipment for new students/postdocs and research technicians will be conducted quarterly, an email will be sent to the flow-core-l email list and the schedule will be posted. The training class will cover the basics of flow cytometry and an introduction to the use of the instrument and software analysis of data. All new users will be required to take this course before they are allowed to use the equipment. Any user inactive for six months will be required to repeat the course as policies may change. One-on-one training and operator assisted use of the equipment is available for interested users and may be required for independent access to the facility. Individual training/operator assisted use can be scheduled with the facility director. Access to and independent use of the facility will be at the discretion of the facility director and can be revoke upon improper use of the facility and failure to comply with facility policies.

Flow Cytometry Educational Resources
From BD Biosciences:

  • Introduction to Flow Cytometry and FACSDiva 6.0 tutorials.
  • Tools for developing a flow cytometry experiment including a spectrum viewer of commonly used fluorochromes and a multicolor panel designer.

Reservations and requests for sorting and analysis are now in CORUM.

Sign Up Policy
Users of the LSRII will be able to sign up on a first come/first serve basis however you will not be able to sign up for more than 3 hours without prior permission from the flow facility manager, Amy Burnside. Additionally, users are asked not to sign up more than 2 weeks in advance without permission from the facility manager. Sign up for LSR II analysis with operator assistance or cell sorting with the FACSAria require contacting the facility manager by email, in person or by telephone at 413-545-1385. Before considering a sorting experiment, the facility manager must be contacted and details of the experiment discussed. Please note that sorting of human and non-human primate material will require prior discussion with the facility manager.

Cancellation Policy
Cancellations on the LSR II must be done 24 hours in advance and a charge of 50% of the sign up time will be billed for cancellations less than 24 hours in advance. Cancellations on the FACSVantage or FACSAria cell sorter must be done 48 hours in advance and a 4 hour charge will be billed for late cancellations if the time is not reassigned to another user.

We would like to thank Prof. Lisa Minter, VASCI, and Heather Sherman, MCB, for providing the header image.