State-of-the-Art Instrumentation Support for Next-Generation Sequencing Services
Equipment
Illumina MiSeq Sequencing System
Mid-throughput sequencer, capable of generating up to 15 Gb data with 50 million paired-end sequencing reads with 300 bp read length. Applied for applications such as 16S rRNA amplicon sequencing, targeted gene sequencing, small genome sequencing and targeted gene expression.
Bioruptor Pico Sonicator System
Sonication device for shearing DNA and RNA for NGS applications. Optimized for volumes of 20 - 100 μl.
BluePippin, Sage Science
DNA size selection system, capable of selecting to 100 bp – 50 kb for NGS applications. Has integrated pulsed-field electrophoresis for resolving and collecting high molecular weight DNA for PacBio library prep as well.
Agilent 2100 Bioanalyzer
Used for sizing, quantitation and quality control of DNA, RNA, and proteins.
FastPrep-24 5G Sample Homogenizer (MP Biomedicals)
An advanced, high-speed benchtop homogenizer used for lysis of any type of cells, tissues, bacteria, fungi, plants, soil samples, etc.
Thermo Scientific Savant DNA120 SpeedVac
High-performance concentrator for efficient concentration, and drying of small-volume DNA or RNA samples.
Bio-RAD CFX96 Touch Real-Time PCR Detection System
The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions.
10x Genomics Chromium Controller
The Chromium Controller uses advanced microfluidics to perform single cell partitioning and barcoding in a matter of minutes. Powered by Next GEM technology, the Chromium Controller enables integrated analysis of single cells at massive scale.
Nexcelom Cellometer K2 Fluorescent Cell Counter
The Nexcelom Cellometer K2, powered by Matrix software, utilizes brightfield imaging and dual-fluorescence imaging to quickly and accurately identify and count individual cells. Cell count, concentration, diameter, and % viability are automatically calculated and reported.
Qubit 4 Fluorometer
The Invitrogen Qubit 4 Fluorometer accurately and quickly measures the concentration of DNA, RNA, or protein in a single sample. It can also be used to assess RNA integrity and quality. The easy-to-use touchscreen menus make it easy to select and run the assays you need, with results displayed in just a few seconds.
Other Services
GRL maintains Supply Center and Freezer Program located in Morrill 4, N214. Bio-Rad, New England Biolab.
GRL and Integrated DNA Technologies (IDT) have created a portal for Discounted Primer orders.
Staff
Location
N330 Morrill I
University of Massachusetts Amherst
637 North Pleasant Street
Amherst, MA 01003
413-545-3848