Genomics Resource Laboratory


Genomics Resource Laboratory

State-of-the-Art Instrumentation Support for Next-Generation Sequencing Services

Located on the 3rd floor in the Morrill Science Center the Genomics Resource Laboratory (GRL) provides services and advanced instrumentation support for nucleic acid (DNA and RNA) analysis.

The GRL provides a suite of services to address your high-throughput next-generation sequencing, including solutions for sample processing such as nucleic-acid isolation, nucleic-acid quantitative and qualitative analysis, NGS library preparation, quantitative-PCR analysis, etc.

We provide a sample processing and library preparation such as whole genome sequencing, shotgun metagenomics, metatranscriptomics, targeted amplicon sequencing, RNA-Seq, ChIP-Seq, Exome Sequencing, Methyl-seq, Single Cell Genomics, etc., to address your NGS projects.

The facility accepts samples and will perform requested analysis. We offer training to users to conduct experimentation for use on a fee for service basis to both internal and external researchers, academic or industry based.

Following an initial consultation, covering experimental parameters training and access is arranged through the director.

What Makes Us Unique

  • Library preparation
    • Whole genome sequencing
    • Shotgun metagenomics
    • Metatranscriptomics
    • 16S/18S rRNA amplicon library prep
    • RNA-Seq
    • ChIP-Seq
    • Other library preps
  • DNA and RNA isolation

Equipment

Illumina MiSeq Sequencing System

Illumina MiSeq Sequencing System

Mid-throughput sequencer, capable of generating up to 15 Gb data with 50 million paired-end sequencing reads with 300 bp read length. Applied for applications such as 16S rRNA amplicon sequencing, targeted gene sequencing, small genome sequencing and targeted gene expression.

Bioruptor Pico Sonicator System

Bioruptor Pico Sonicator System

Sonication device for shearing DNA and RNA for NGS applications. Optimized for volumes of 20 - 100 μl.

BluePippin, Sage Science

BluePippin, Sage Science

DNA size selection system, capable of selecting to 100 bp – 50 kb for NGS applications. Has integrated pulsed-field electrophoresis for resolving and collecting high molecular weight DNA for PacBio library prep as well.

Agilent 2100 Bioanalyzer

Agilent 2100 Bioanalyzer

Used for sizing, quantitation and quality control of DNA, RNA, and proteins.

FastPrep-24 5G Sample Homogenizer (MP Biomedicals)

FastPrep-24 5G Sample Homogenizer (MP Biomedicals)

An advanced, high-speed benchtop homogenizer used for lysis of any type of cells, tissues, bacteria, fungi, plants, soil samples, etc.

Thermo Scientific Savant DNA120 SpeedVac

Thermo Scientific Savant DNA120 SpeedVac

High-performance concentrator for efficient concentration, and drying of small-volume DNA or RNA samples.

 Bio-RAD CFX96 Touch Real-Time PCR Detection System

Bio-RAD CFX96 Touch Real-Time PCR Detection System

The CFX96 Touch System is a powerful, precise, and flexible real-time PCR detection system. This six-channel (five colors and one FRET channel) real-time PCR instrument combines advanced optical technology with precise temperature control to deliver sensitive, reliable detection for singlexplex or multiplex reactions.

10x Genomics Chromium Controller

10x Genomics Chromium Controller

The Chromium Controller uses advanced microfluidics to perform single cell partitioning and barcoding in a matter of minutes. Powered by Next GEM technology, the Chromium Controller enables integrated analysis of single cells at massive scale.

Nexcelom Cellometer K2 Fluorescent Cell Counter

Nexcelom Cellometer K2 Fluorescent Cell Counter

The Nexcelom Cellometer K2, powered by Matrix software, utilizes brightfield imaging and dual-fluorescence imaging to quickly and accurately identify and count individual cells. Cell count, concentration, diameter, and % viability are automatically calculated and reported.

Qubit 4 Fluorometer

Qubit 4 Fluorometer

The Invitrogen Qubit 4 Fluorometer accurately and quickly measures the concentration of DNA, RNA, or protein in a single sample. It can also be used to assess RNA integrity and quality. The easy-to-use touchscreen menus make it easy to select and run the assays you need, with results displayed in just a few seconds.

Other Services

GRL maintains Supply Center and Freezer Program located in Morrill 4, N214. Bio-Rad, New England Biolab.
GRL and Integrated DNA Technologies (IDT) have created a portal for Discounted Primer orders.

Staff

  • Location

    N330 Morrill I
    University of Massachusetts Amherst
    637 North Pleasant Street
    Amherst, MA 01003

    413-545-3848

    Genomics Resource Laboratory Directions