Animal Models


Animal Models

Provides transgenic, gene targeting, and other services to the research community. The core performs microinjections of DNA into fertilized embryos to generate transgenic mice. Uses cutting-edge technologies, including CRISPR/Cas9 genome editing, to generate gene knock-out or knock-in mice or other animal models. In addition, provides mouse surgery service and training, such as patient-derived xenograft (PDX), tail vein injection, and catheterization. The core is able to customize services as requested to support development of animal models of human diseases in a timely and cost-efficient manner.

Remote video URL
Watch Core Facilities Seminar-Animal Models on YouTube.
Tuesday, May 17, 2022

Animal Models

2:00 pm

Wei Cui, Director of Animal Models and Extension Assistant Professor Veterinary & Animal Sciences–"Learn about the services provided through the Animal Models Core Facility."

Equipment

Eppendorf TransferMan®4r Micromanipulators

Eppendorf TransferMan®4r Micromanipulators

These manipulators possess an ergonomic and innovative design to meet the most demanding micromanipulation requirements. Smart functions, such as improved automated home function and position storage, enable simple and fast execution of even the most complex micromanipulation techniques. The DualSpeed™ joystick allows for precise and intuitive movement in all three dimensions, and dynamic movement for covering longer distances. It is ideal for manipulation of oocytes and early embryos, transfer of stem cells, and a wide range of applications in reproductive biology.

Eppendorf CellTram®4r Air/Oil Microinjectors

Eppendorf CellTram®4r Air/Oil Microinjectors

These microinjectors are manual microinjectors suitable for a broad range of applications in reproductive biology. The CellTram 4r Air is a pneumatic injector for oil-free micromanipulation and gentle holding of oocytes/embryos in suspension. The CellTram 4r Oil is a hydraulic, oil-filled microinjector that provides more direct sample control and can generate higher pressures than an air system.

Eppendorf PiezoXpert®

Eppendorf PiezoXpert®

Allow for piezo-assisted micromanipulation, which improves the results of a variety of applications including transfer of embryonic or induced pluripotent stem cells into blastocysts and morulae, injection into mammalian oocytes and zygotes, and enucleation/nuclear transfer.

Eppendorf Galaxy 48R Incubator

Eppendorf Galaxy 48R Incubator

Features a large backlit display with on screen help menu, control and data logging of temperature, CO2, door openings, alarms, as well as O2 gas and humidity monitoring.

Thermo HERA150i Incubator

Thermo HERA150i Incubator

With Stainless-Steel Chamber-provides an ideal in-vitro environment. It features fast recovery characteristics and a convenient touchscreen user interface.

Nikon Ts2R Inverted Microscope

Nikon Ts2R Inverted Microscope

A new standard for the inverted research microscope, providing a wide range of observation methods and applications in a compact body with four fluorescence channels available.

Nikon SMZ-800N Stereoscopes

Nikon SMZ-800N Stereoscopes

Offer higher magnification than conventional models, enabling high-resolution observation. In combination with the new objective series, its improved chromatic aberration correction provides bright and sharp images through the entire viewfield.

DeNovix DS-11 Spectrophotometer

DeNovix DS-11 Spectrophotometer

A compact instrument that delivers full spectrum UV-Vis analysis and is ideal for nucleic acid and protein quantification

Sutter P-1000 Micropipette Puller

Sutter P-1000 Micropipette Puller

Features include: Color touch screen display; Safe heat mode to protect and extend filament life; Pipette Cookbook program directory; Pre-heat mode improves stability; Line repeat mode simplifies multi-line programming; Self-contained air supply with filtration system and humidity control chamber.

Narishige MF-900 Microforge

Narishige MF-900 Microforge

Offers a wide range of functions such as cutting, bending, fire-polishing, and forming a spike for making many different kinds of glass pipette tips.

Thermo Ultra-Low TSX freezers

Thermo Ultra-Low TSX freezers

Designed to meet the highest standards in reliability, sustainability and temperature management. At the heart of the TSX Series is V-drive technology, designed to adapt to user patterns, substantially reducing energy and HVAC costs without compromising performance.

Syngene InGenius3 Gel Documentation

Syngene InGenius3 Gel Documentation

A gel documentation system for imaging DNA and RNA. This gel imaging system features a compact darkroom with a slide-out transilluminator and an internal LED white light.

Staff