The University of Massachusetts Amherst
Cell Culture

Cell Culture

Announcements

Core Facilities Seminar
September 20, 2-3 p.m., Meet & Greet following
LSL S330-340

"Kinetic Stem Cell (KSC) Counting - a new technology for routine determination of the specific fraction or dosage of therapeutic tissue stem cells," James L. Sherley, MD, PhD, President & CEO, Asymmetrex® LLC

The Cell Culture Facility (CCF) located in the Life Science Laboratories supports research by providing space, equipment, cell cultures (plant and mammalian), media, supplies and training on a recharge basis for both academic labs and corporate partners. The Facility also offers technical expertise and hands on support for custom research projects. Labs are equipped with laminar flow hoods, incubators, centrifuges, refrigerators/freezers, basic microscopes and automated cell counters.

The Plant Cell Culture Library (PCCL) is a globally unique collection of callus cultures representing more than 1,000 diverse plant species.

Starter cultures and growth media may be purchased from the Collection for academic or commercial research with no IP or licensing restrictions.
 

Plant Cell Culture Library (PCCL)

The Plant Cell Culture Library (PCCL) at UMass Amherst is a globally unique collection of callus cell cultures representing more than 1,000 diverse plant species.  Plants produce over 200,000 different kinds of small organic chemicals called “secondary” or “specialized” metabolites that serve many important roles in plant biology, and have been used in various industries including pharmaceuticals, cosmetics, flavors, dyes, dietary supplements and fragrances.
 
This unique living resource is available to the entire research community to discover new biological pathways or new methods for plant based product development.

Starter cultures and growth media may be purchased from the Collection for academic or commercial research with no IP or licensing restrictions.

Search the Collection at umass.edu/ials/pccl-database.

Mammalian Cell Culture (LSL S570)
  • 2 BSL2 Biosafety cabinets
  • 2 Heracell VIOS 160i Incubators
  • 4°C and -20°C storage
  • Inverted Nikon phase contrast microscope
  • Centrifuge
  • Countess FLII automated cell counter (red/green fluorescence capability)
  • CELLINK Bio X 3-D Bioprinter
  • Common consumables and media on re-charge basis
     

General Cell Culture (S471)
  • 2 BSL2 Biosafety cabinets
  • 2 Heracell VIOS 160i Incubators (1 with low oxygen capability)
  • Refrigerator
  • -20 Freezer
  • -80 Freezer
  • Liquid nitrogen storage
  • Inverted Nikon phase contrast microscope
  • Common consumables and media on re-charge basis
     

Plant Cell Culture (LSL S579)
  • 2 BSL2 Biosafety cabinets
  • Germinator 500 instrument sterilizer

Media prep station, with

  • Barnstead EASYpure water filtration system
  • Analytical balance and bench scale
  • Magnetic stir plates
  Campus Users Other Academic Institutions External Users
Facility Access Fee $250/quarter $325/quarter $500/quarter
Facility Access Fee-Additional Lab Member $50/quarter $65/quarter $100/quarter
Plate Cell Culture (Simple) $100/plate $130/plate $200/plate
Plate Cell Culture (Medium complex)) $200/plate $260/plate $400/plate
Plate Cell Culture (Difficult) Inquire Inquire Inquire
Plate Cell Culture Extracts $25/aliquot $32.50/aliquot $50/aliquot
Trans-Well/Co-Culture
40 cell lines in duplicate
$125/well $162.50/well $250/well
Agar Diffusion $125/diffusion $162.50/diffusion $250/diffusion
Technican Time $75/hour $75/hour $150/hour
 
Rates are subject to change and do not include shipping, contact facility to verify current fees.

FY22-23 Specialized Service Center Approved Fees

Services

    Cell Culture Facility: The equipment in this facility allow for routine mammalian cell culture work and includes incubators, hoods, refrigeration, centrifuges, microscopes, pipettes, etc. This facility allows for walk-in usage by users and will soon provide services including cell passage, storage, transfection, etc.

    FY22-23 Specialized Service Center Approved Fees

    March 2022

    Great ideas, but no lab?  We can do the wet work for you!

    • Cell Line expansion and cryopreservation
    • Stem cell culture
    • Liquid nitrogen storage
    • Assay Development/optimization
    • CRISPR based gene editing and stable clone selection
    • Mycoplasma testing
    • Lentiviral packaging, titer, transduction
    • Molecular biology

    Questions about something different? Ask us!
    We also offer consultation to help you move your research forward.