In a first-of-its-kind research breakthrough, a team of UMass scientists including the Center for Bioactive Delivery's (CBD) Leonid Pobezinsky and Elena Pobezinskaya have analyzed and described what they call the “mosquito effect,” which sheds light on how certain pathogens, such as cancerous tumor cells, can outwit the body’s immune system.
Just as mosquitoes ingest their host’s blood, the immune system’s T cells incorporate cytoplasmic material from tumors into their own cytoplasm. While it has long been known that many kinds of cells can transfer cellular material from one to another, the transfer of the cytoplasm has never been observed in T cells. Subsequent single-cell RNA (scRNA) sequencing shows that cytoplasm from tumor cells alters the machinery responsible for protein coding in the host T cell. The research, reported recently in the journal Frontiers in Immunology, is a major step forward in understanding how tumors can successfully evade the immune system, and thus a step toward more effective treatments.
One of the great mysteries in medicine is how certain pathogens can suppress the immune system in order to spread wildly. There are many different parts to the immune system, but among the most important are T cells, which identify and attack pathogens, and the T regulatory cells, which tell the T cells when it’s safe to call off the attack, limiting collateral damage to the body.
And yet, cancerous tumor cells have figured out how to short-circuit the immune system, with often catastrophic results for healthy tissues. How, exactly, tumor cells do this is unknown, but, says Leonid Pobezinsky, member of CBD, associate professor of veterinary and animal sciences at UMass Amherst, and the paper’s senior author, “we’ve observed for the very first time that T cells and T regulatory cells suck up a bit of tumor cytoplasm and integrate it into their own.”
To make the discovery, Pobezinsky and his team, including Elena Pobezinskaya, also a member of CBD, research assistant professor in veterinary and animal sciences at UMass, and co-senior author of the paper, engineered tumor cells to produce an ultrabright fluorescent protein called ZsGreen. They then introduced the green-glowing tumor cells into a mouse model. After eight days, the model’s tumor-infiltrating immune cells were gathered and analyzed using state-of-the-art equipment in the Flow Cytometry lab at UMass Amherst’s Institute for Applied Life Sciences.
"What we saw was striking,” says Pobezinskaya. “The T cells were glowing and uniform green, which tells us that the tumor’s cytoplasm had been distributed widely throughout the T cell.”
Even more surprising was to see the T regulatory cells light up as well. And the team found that the cells glowing the brightest were the ones most exhausted from their fight against the tumor. Finally, the team determined that the transference of cellular material requires the cells of the tumor and the immune system to come into physical contact with each other.