Lynne A. McLandsborough | Department of Food Science

Professor, and Department Head

Field of Study:

Food safety, cleaning and sanitation, biofilm formation and removal; antimicrobial delivery systems

Office:

Room 241, Chenoweth Laboratory

Telephone:

(413) 545-1016

Email:

lm@foodsci.umass.edu

Details

Research

In general bacteria tend to accumulate at interfaces between two phases in heterogeneous systems. As long as water is available for microbial growth, microorganisms can be found foods and processing environments at solids-liquid, gas-liquid, and solid-gas interfaces. In their natural environments, bacteria do not exist as isolated cells but grow and survive in organized communities on surfaces. These communities are called biofilms and can be simplistically defined as bacterial growth on a solid surface. When growing in a biofilm, bacteria are known to have different growth rate, morphology, and physiology than their planktonic counterparts and may exhibit varied physiological responses to nutrient conditions resulting in increased resistance to antimicrobials agents compared with their planktonic forms.

Multispecies biofilms within food processing environments are a major source of L. monocytogenes in processed foods. When growing on surfaces, this organism exhibits enhanced resistance to conventional chemical sanitizers, germicides and heat making control even more challenging.

My laboratory is interested in multiple aspects of biofilms:

The genetics of Listeriasp. biofilm growth.
The biological, physical and chemical aspects of bacterial adhesion, transfer and removal.
Antimicrobial delivery systems for cleaning and sanitation

Teaching

Fd Sci 150 -The Science of Food (4 cr) Co-Instructor

Awards

Finalist, 2018-2019 UMass Distinguished Teaching Award

College of Food and Natural Resources Certificate for Excellence in Teaching, December 2000

College of Food and Natural Resources Outstanding Teacher Award, Dec 2001

College of Food and Natural Resources Outstanding Advisor Award

Publications

Peer-Reviewed Publications

Goshal, M, S. Chuang, Y. Zhang, and L. McLandsborough. 2022. Efficacy of acidified oils against Salmonella in low-moisture environments. Pre-print https://doi.org/10.1128/aem.00935-22*
*Selected for ASM press release 8/8/2022

Ghoshal, M., V. Ryu, and L. McLandsborough. 2022. Evaluation of the efficacy of antimicrobials against pathogens on food contact surfaces using a rapid microbial log reduction detection method. Intl J. Food Microbiol 373:109699

Ryu, V., Ruiz-Ramirez, S., Chuesiang, P., McLandsborough, L.A., McClements, D.J. and Corradini, M.G., 2021. Use of Micellar Delivery Systems to Enhance Curcumin’s Stability and Microbial Photoinactivation Capacity. Foods, 10:1777.

Chuesiang, P., Ryu, V., Siripatrawan, U., McLandsborough, L. and He, L., 2021. Investigation of factors that impact the label-free surface-enhanced Raman scattering (SERS) for the detection and discrimination of Salmonella Enteritidis. LWT, p.111962.

Chuesiang, P., Ryu, V., Siripatrawan, U., He, L. and McLandsborough, L., 2021. Aptamer-based surface enhanced Raman spectroscopy (SERS) for the rapid detection of Salmonella Enteritidis contaminated in ground beef. LWT, p.111937.

Lane, K., McLandsborough, H, L. A., Autio, W. R., & Kinchla, A. J., 2020. Efficacy of ATP Monitoring for Measuring Organic Matter on Postharvest Food Contact Surfaces. J Food Prot. 83:1829-1837.

Ryu, V., M. Corradini, D. J. McClements, L. McLandsborough. 2019. Impact of ripening inhibitors on molecular transport of antimicrobial components form essential oil nanoemulsions. J. Coll. Int. Sci. 556:568-576

Hung, Y-T, L. A. McLandsborough, J. M. Goddard and L. J. Bastarrachea. 2018. Antimicrobial polymer coating with efficacy against pathogenic and spoilage microorganisms. LWT-Food Sci Tech. 97:546-554.

Ryu, V, D. J. McClements, M. G. Corradini, J. S. Yang and L. McLandsborough, 2018. Natural antimicrobial delivery systems: formation, antimicrobial activity, and mechanism of action of quillaja saponin-stablilized carvacrol nanoemulsions. Food Hydrocolloids. 82:442-450.

Chuesiang P, U. Siripatrawan, R. Sanguandeekul. L. McLandsborough. J. D. McClements. Optimization of cinnamon oil nanoemulsions using phase inversion temperature method: Impact of oil phase composition and surfactant concentration. J. Col. Interf. Sci. 154:208-216

Pearson B, Mills A, Tucker M, Gao S, McLandsborough L, He L. 2018. Rationalizing and advancing the 3-MPBA SERS sandwich assay for rapid detection of bacteria in environmental and food matrices. Food Microbiology 72:89-97.

Ryu V, McClements DJ, Corradini MG, McLandsborough L. 2018. Effect of ripening inhibitor type on formation, stability, and antimicrobial activity of thyme oil nanoemulsion. Food Chemistry 245:104-111

Landry, K. S., Sela, D. A., & McLandsborough, L. (2018). Influence of sprouting environment on the microbiota of sprouts. Journal of Food Safety. DOI: 10.1111/jfs.12380

Wang, L. J. G. Stoffolano and L. McLandsborough. 2017. Development of the fly “crop vessel’ bioassay for fly/microbial studies. Afr. J. Microbiolol. Res. 11:1027-1034

Pearson, B., Wang, P., Mills, A., Pang, S., McLandsborough, L., & He, L. (2017). Innovative sandwich assay with dual optical and SERS sensing mechanisms for bacterial detection.Analytical Methods, 9(32), 4732-4739.

Wang, P., Pang, S., Pearson, B., Chujo, Y., McLandsborough, L., Fan, M. and He, L., 2017. Rapid concentration detection and differentiation of bacteria in skimmed milk using surface enhanced Raman scattering mapping on 4-mercaptophenylboronic acid functionalized silver dendrites. Anal Bioanal Chem., 409:2229-2238.

Landry, S. K., J. Komaiko, D. Wong, T. Xu, D. J. McClements and L. McLandsborough. 2016 The inactivation of Salmonella spp. on sprouting seeds using a spontaneous carvacrol nanoemulsion acidified with organic acids. J. Food Protection 79:1115-1126.

Huang, K., L. A. McLandsborough, and J. M. Goddard. 2016. Adhesion and removal kinetics of Bacillus cereus biofilms on Ni-PTFE modified stainless steel. Biofouling 32:523-533

Wang P, S. Pang J. Chen, L. McLandsborough, S.R .Nugen, M. Fan, and L. He. 2016. Label-free mapping of single bacterial cells using surface-enhanced Raman spectroscopy. Analyst 141:1356-1362

Landry, K. S., S. Micheli, D. J. McClements and L. McLandsborough. 2015, Effectiveness of a spontaneous carvacrol nanoemulsion against Salmonella enterica Enteritidis and Escherichia coli O157:H7 on contaminated broccoli and radish seeds. J. Food Microbiology. 51:10-17.

Chang, Y., L. McLandsborough, D. J. McClements. 2015. Fabrication, Stability and efficacy of dual component antimicrobial nanoemulsions: essential oil (thyme oil) and cationic surfactant (lauric arginate). Food Chem. 172:298-304.

Landry, K. S., Y. Chang, D. J. McClements, and L. McLandsborough. 2014. Effectiveness of a novel spontaneous carvacrol nanoemulsion against Salmonella enterica Enteritidis and Escherichia coli O157:H7 on contaminated mung bean and alfalfa seeds. Intl. J. Food Microbiol. 187:15-21.

Loeffler, M., D. J. McClements, L. McLandsborough, N. Terjung, Y. Chang, and J. Weiss. 2014. Electrostatic interactions of cationic lauric arginate with anionic polysaccharides affect antimicrobial activity against spoilage yeasts. J. Appl. Microbiol. 117:28-39.

Talbert, J., K. Seto, J. Cotter, L. McLandsborough, J. M. Goddard. 2014. Effect of cleaning and sanitizing agents on the surface characteristics of new and extended-wear produce picking bins. J. Sci of Food and Agr. 94:1681-1687

Bastarrachea, L.J., L.A. McLandsborough, M. Peleg, J. M. Goddard. 2014 Antimicrobial N-halamine modified polyethylene, characterization, biocidal efficacy, regeneration, and stability. J. Food Sci. 79:E887-E897.

Chang, YH, L. McLandsborough, D. J. McClements. 2014. Interaction of cationic antimicrobial (ε-polylisine) with food-grade biopolymers: dextran, chitosan, carrageenan, alginate, and pectin. Food Res. Intl. 64:396-401

Chang, YH, L. McLandsborough, D. J. McClements. 2014. Antimicrobial delivery systems based on electrostatic complexes of cationic ε -polylysine and anionic gum arabic. Food Hydrocolloids. 35:137-143.

Bastarrachea, LJ, M. Peleg, L. McLandsborough and J. M Goddard. 2013. Low density polyethylene modified with antimicrobial N-halamines: Kinetics of inactivation against Listeria monocytogenes and N-halamine regeneration. J. Food Eng. 117:52-58.

Chang, YH, WM Gu, FJ Zhang, and L. McLandsborough. 2013. Disruption of lmo1386, a putative DNA translocase gene, affects biofilm formation of Listeria monocytogenes on abiotic surfaces. Intl. J. Food Microbiol. 161:158-163.

Chang, Y. L. McLandsborough. And D. J. McClements. 2012. Physical properties and antimicrobial efficacy of thyme oil nanoemulsions: influence of ripening inhibitors. J. Ag. Food Chem. 60:12056-120563.

Y. Chang, W. Gu, N. Fischer and L. McLandsborough. 2012. Identification of genes involved in Listeria monocytogenes biofilm formation by mariner-based transposon mutagenesis. Appl. Microbiol. Biotechnol. 93:2051-2062 DOI10.1007/s00253-011-3719-z. Published on line Nov 27,2011.

Y. Chang, L. McLandsborough 2012 Low Concentration of Ethylenediaminetetraacetic Acid (EDTA) Affects Biofilm Formation of Listeria monocytogenes by Inhibiting its Initial Adherence. Food Microbiology . 29:10-17

Chang, Y. H., L. McLandsborough, D. J. McClements. 2012. Cationic Antimicrobial (epsilon-Polylysine)-Anionic Polysaccharide (Pectin) Interactions: Influence of Polymer Charge on Physical Stability and Antimicrobial Efficacy. J. Ag. Food Chem. 60:1837-1844

Y. Chang, L. McLandsborough, D. J.McClements. 2011. Physiolochemical properties and antimicrobial efficacy of electrostatic complexes based on cationionic epsilon-polylysine and anionic pectin. J. Agricultural and Food Chemistry. 12:6776-6782.

K. Ziani, Y. Chang, L. McLandsborough, D. J. McClements. 2100. Influence of surfactant charge on antimicrobial efficacy of surfactant-stabilized thyme oil nanoemulsions. J. Agriculture and Food Chemistry 11: 6247-6855

Y. Chang, L. McLandsborough, D. J. McClements 2011. Interactions of a cationic antimicrobial (epsilon-Polylysine) with an anionic biopolymer r(pectin): an isothermal titration calorimetry, microeletophoresis and turbidity study. J. Agricultural and Food Chemistry 10:5579-5588.

D. Pérez-Conesa,, J. Cao, L. Chen, L. McLandsborough^,and J. Weiss^. 2011 Inactivation of Listeria monocytogenes and Escherichia coli O157:H7 Biofilms by Micellar-Encapsulated Eugenol and Carvacrol J. Food Protection 74:55-62

Roberts, A. J., J. Robbins, L. McLandsborough, and M. Wiedmann. 2010. A 10 Year Review of the Food Science Summer Scholars Program at Cornell University and the University of Massachusetts: A Model for Research Training and for Recruiting Undergraduate Students into Graduate Programs and Careers in Food Science J. Food Sci Edu. 9:98-105.

Dai Y, L A. McLandsborough, J.Weiss, and M. Peleg^. 2010 The concentration and application order effects of sodium benzoate and eugenol mixtures on the growth inhibition if Saccharomyces cereviceae and Zygosaccharomyces bailii. J. Food Sci 75: M482-M488

Rodríguez, A., W. R. Autio and L. A. McLandsborough.2008. Effects of contact time, pressure, percent relative humidity (%RH) and material type on Listeria biofilm adhesive strength at a cellular level using atomic force microscopy (AFM). Food Biophyics. 3:305-311.

Rodríguez, A., W. R. Autio and L. A. McLandsborough. 2008 Effect of surface roughness and stainless steel finish on Listeria monocytogenes attachment and biofilm formation. J. Food Protection 71:170-175.

Rodríguez, A., W. R. Autio and L. A. McLandsborough. 2007. Effect of biofilm dryness on the transfer of Listeria monocytogenes biofilms grown on stainless steel to bologna and hard salami. J. Food Protection 70 : 2480-2484

P.A. Beffa-Negrini, N, L. Cohen, M. J. Laus, and L. McLandsborough. 2007. Development and Evaluation of an online, inquiry based food safety education program for secondary teachers and their students. J. Food Science Education. 6:66-71

Rodríguez, W. R. Autio and L. A. McLandsborough. 2007. Effects of inoculation level, material hydration, and stainless steel surface roughness on the transfer of Listeria monocytogenes from inoculated bologna to stainless steel and high density polyethylene J. Food Protection 70:1423-1428

Rodríguez and L. A. McLandsborough 2007. Evaluation of the transfer of Listeria monocytogenes from surfaces to foods. J. Food Protection 70:600-606.

D. Pérez-Conesa, L. McLandsborough, and J. Weiss 2006. Inhibition and Inactivation of Listeria monocytogenes and Escherichia coli O157:H7 Colony Biofilms by Micellar-Encapsulated Eugenol and Carvacrol. J. Food Protection. 69: 2947-2954

Cao, J., C. Cronin, M. Clarke, R. Witkowsky, H. Lu, A. Sayedahaman, R.E. Levin, and L. A. McLandsborough. 2006 Levels and Tracking of Listeria monocytogenes Strains in a Seafood-Processing Environment using Enrichment Most Probable Number and Randomly Amplified Polymorphic DNA. J. Food Prot. 69:489-494

L. McLandsborough, A. Rodriguez, and D. Pérez-Conesa and J. Weiss. 2006. Biofilms: At the Interface between Biophysics and Microbiology. Food Biophysics. 1:94-114

Cao, J, C. Cronin, L. McLandsborough, R. E. Levin. 2005. Effects of primers and Taq polymerase on randomly amplified polymorphic DNA analysis for typing Listeria monocytogenes from the environment of a shrimp processing plant. Food Biotechnol. 19:217-226.

P. Prachaiyo and L. A. McLandsborough. 2003. Oil-in-water emulsion as a model system to study the growth of E. coli O157:H7 in a heterogenous food system. J. Food Sci. 68:1018-1024

D. Djordjevic, M. Wiedmann, and L. A. McLandsborough. 2002. Microtiter plate assay for assessment of Listeria monocytogenes biofilm formation. Appl. Environ. Microbiol. 68:2950-2958.

Li, J., D. J. McClements and L. A. McLandsborough. 2001. Interaction between emulsion droplets and Escherichia coli cells. J. Food Sci. 66:570-575.

Shaw, W.K., and L. A. McLandsborough. 2000. PCR reaction parameter titration as an approach to develop shortened reaction times in a conventional thermal cycler. J. Rapid Meth. Automat. Microbiol. 8:53-64

Prachaiyo, P., and L. McLandsborough. 2000. A microscopic method to visualize Escherichia coli interaction with beef muscle. J. Food Prot. 63:427-433

Fernec, J., J. Oliver, R. Witkowski, L. McLandsborough, and R. Levin. 2000. Studies in the growth of Escherichia coli O157:H7 strains at 45.5°C. J. Food Prot. 63:1173-1178.

Li, J. and L. A. McLandsborough. 1999. The effects of the surface charge and hydrophobicity of Escherichia coli in its adhesion to beef muscle. Int. J. Food Microbiology. 53:185-193

Cleary, P. P., L. McLandsborough, L. Ikeda, D. Cue, J. Krawczak, and H. Lam. 1998. High-frequency intracellular infection and erythrogenic toxin A expression undergo phase variation in M1 group A streptococci. Mol. Microbiol. 28:157:67.

McLandsborough, L. A., L. Sechard, L. L. McKay. 1998. Synergistic effect of combination of lactococcal phage resistance fragments of pNP40 with cloned abortive infection gene abiD. J. Dairy Sci. 81:362-368.

Ji, Y., L. McLandsborough, A. Kondagunta, and P. P. Cleary. 1996. C5a peptidase alters clearance and trafficking of group A streptococci by infected mice. Infect. Immun. 64:503-510.

Cleary, P. P., L. A. McLandsborough, and K. Hoikka Prichard. 1995. Differential expression of genes in the vir regulon. Dev. Biol. Stand. 85:145-8

McLandsborough, L. A. and P. P. Cleary. 1995. Insertional inactivation of virR in Streptococcus pyogenes M49 demonstrates that VirR functions as a positive regulator of streptococcal C5a peptidase and M protein in OF⁺ strains. Dev. Biol. Stand. 85:149-52

McLandsborough, L. A., and P. P. Cleary. 1995. Insertional inactivation of virR in Streptococcus pyogenes M49 demonstrates that VirR functions as a positive regulator of ScpA, FcRA, OF, and M protein. FEMS Microbiol. Let. 128:45-52.

McLandsborough, L. A., K. M. Kolaetis, T. Requena, and L. L. McKay. 1995. Cloning and characterization of the abortive infection genetic determinant abiD isolated for pBF61 of Lactococcus lactis subsp. lactis KR5. Appl. Environ. Microbiol. 61:2023-2026.

McLandsborough, L. and S. R. Tatini. 1991. A 6 h microslide immunodiffusion assay for confirmed detection of staphylococcal enterotoxins. Let. Appl. Microbiol. 12:81-84.

Books

L. McLandsborough. Food Microbiology Laboratory. CRC Press, Boca Raton, FL. 2005.

W. K. Shaw Jr and L. McLandsborough. Instructor’s guide to Food Microbiology Laboratory. CRC Press, Boca Raton, FL. 2005

Book Chapters

L. McLandsborough. 2015. Ch.1. Current knowledge and perspectives on biofilm formation and remediation. In Biofilms in the Food Environment. 2^nd Edition. A. Pometto and A. Demirci, Editors. John Wiley and Sons

L. McLandsborough. 2013. Ch. 27. Microbial Biofilms and Food Safety. In Guide to Foodborne Pathogens. Ed. R. G. Labbe and S. Garcia. John Wiley & Sons. Oxford, UK

Rodríguez-Lozano, A. and L. McLandsborough. 2009. Biofilm formation by Listeria monocytogenes and transfer to foods. In Biofilms in the food and beverage industries. P. M. Fratamico, B. A. Annous and N. W. Gunther, Editors. Woodhead Publishing Limited, Cambridge. P 200-225

Shaw, W. K. Jr. and L. .McLandsborough 2006. Biofilm production by Listeria monocytogenes. In Food Biotechnology 2^nd Edition K. Shetty, G. Paliyath, A. Pometto and R. E. Levin Editors. CRC Press 1329 – 1342.

Full CV

cv_mclandsborough_sept_2022.pdf