More than one billion people worldwide have access to a piped drinking water system that supplies water intermittently. An intermittent water supply (IWS) system is a piped water system that delivers water to end-users discontinuously, with days or hours of interruption; these systems are correlated with higher rates of waterborne illnesses in populations. Despite piped drinking water distribution systems representing a critical exposure route for disease transmission and spreading antimicrobial resistance (AMR) to opportunistic pathogens, no study has explored the role of intermittency in disseminating antibiotic-resistant genes (ARGs). AMR is associated with an estimated 4.95 million deaths in 2019, and it is estimated to increase to 10 million deaths by 2050. My research aims to understand and investigate the role of frequent interruptions (intermittency) on the prevalence and transmission of antibiotic-resistant genes (ARGs) in piped drinking water networks. Ultimately, this research has the potential to illuminate how IWS systems contribute to the spread of drug resistance and shape policy decisions to improve water systems globally.