Role of mitochondrial metabolism in pathophysiology:
The long-term objectives of my group are to understand how primary impairments of mitochondrial metabolism predispose to disease, and identify the molecular, biochemical, physiological, nutritional and environmental causes of metabolic impairments. Our studies involve a variety of approaches including time-lapse functional imaging (confocal & wide field: ROS, NAD(P)/NAD(P)H, GSH/GSSG, cAMP, Ca2+), in situ respirometry (flow-through, Seahorse Extracellular (XF) Analyzer, & polarography), biochemical (enzyme assays, BN-PAGE, & others), metabolomics (global using tissue & cells), gene expression analyses (microarray, RNASeq, and quantitative PCR), affinity tagging/purifications, and molecular genetics approaches including gene editing (CRISPR/Cas9 & TALENs) to probe mitochondrial function and biogenesis. A special emphasis is given to mitochondrial bioenergetics in intact cells i.e. in situ. The ongoing research projects intersect disease area of cancer, diabetes, neurodegeneration, and mitochondrial diseases.
- Develop metabolic biomarkers for personalized patient care for assessing susceptibility to disease, therapeutic response, and prognosis.
- Develop assays/technology for assessing mitochondrial function under physiological conditions. The XF-PMP product marketed by Seahorse Bioscience is based on a technology in my laboratory. Personally, I have played a significant role in validation and improvement of the Seahorse Bioscience’s XF technology.
- BS Banaras Hindu University, Varanasi, India, 1989
- MS University of Baroda, Vadodara, India, 1991
- PhD Jawaharlal Nehru University, New Delhi, India, 1998
- Postdoctoral Training: Buck Institute for Age Research, 2005-2008; Division of Biological Sciences, University of California San Diego, 1998-2005; Chicago Medical School, 1997-1998