The University of Massachusetts Amherst
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Nagendra Yadava

Adjunct Assistant Professor, Department of Biology; Principal Investigator, Pioneer Valley Life Sciences Institute; Assistant Professor, Tufts University School of Medicine, Baystate Medical Center

Current Research
Role of mitochondrial metabolism in pathophysiology:
The long-term objectives of my group are to understand how primary impairments of mitochondrial metabolism predispose to disease, and identify the molecular, biochemical, physiological, nutritional and environmental causes of metabolic impairments. Our studies involve a variety of approaches including time-lapse functional imaging (confocal & wide field: ROS, NAD(P)/NAD(P)H, GSH/GSSG, cAMP, Ca2+), in situ respirometry (flow-through, Seahorse Extracellular (XF) Analyzer, & polarography), biochemical (enzyme assays, BN-PAGE, & others), metabolomics (global using tissue & cells), gene expression analyses (microarray, RNASeq, and quantitative PCR), affinity tagging/purifications, and molecular genetics approaches including gene editing (CRISPR/Cas9 & TALENs) to probe mitochondrial function and biogenesis. A special emphasis is given to mitochondrial bioenergetics in intact cells i.e. in situ. The ongoing research projects intersect disease area of cancer, diabetes, neurodegeneration, and mitochondrial diseases.

Translational research:

  • Develop metabolic biomarkers for personalized patient care for assessing susceptibility to disease, therapeutic response, and prognosis.
  • Develop assays/technology for assessing mitochondrial function under physiological conditions. The XF-PMP product marketed by Seahorse Bioscience is based on a technology in my laboratory. Personally, I have played a significant role in validation and improvement of the Seahorse Bioscience’s XF technology.

Academic Background

  • BS Banaras Hindu University, Varanasi, India, 1989
  • MS University of Baroda, Vadodara, India, 1991
  • PhD Jawaharlal Nehru University, New Delhi, India, 1998
  • Postdoctoral Training: Buck Institute for Age Research, 2005-2008; Division of Biological Sciences, University of California San Diego, 1998-2005; Chicago Medical School, 1997-1998
Sen S., Domingues C.C., Rouphael R., Chou C., Kim C., Yadava N. (2015) Genetic modification of human mesenchymal stem cells helps to reduce adiposity and improves glucose tolerance test in an obese diabetic mouse model. Stem Cell Res. Ther. in press.
Yadav N., Kumar S., Marlowe T., Chaudhary A.K., Kumar R., Wang J., O’Malley J., Boland P., Jayanthi S., Kumar T.K.S., Yadava N., Chandra D.(2015). Oxidative phosphorylation-dependent regulation of cancer cell apoptosis in response to anticancer agents. Cell Death & Dis., in press
Quinlan C.L., Goncalves R.L.S., Hey-Mogensen M., Yadava N., Bunik V.I., Brand M.D. (2014): The 2-oxoacid dehydrogenase complexes in mitochondria can produce superoxide/hydrogen peroxide at much higher rates than complex I. J. Biol. Chem. 289:8312-8325.
Kim C., Patel P., Gouvin, L.M., Brown, M.L., Khalil, A., Henchey, E.M., Heuck A.P., Yadava N., (2014): Comparative analysis of the mitochondrial physiology of pancreatic cells. Bioenergetics, 3: 110. doi:10.4172/2167-7662.1000110.
Divakaruni A.S., Wiley S.E., Rogers G.W., Andreyev A.Y., Petrosyan S., Loviscach M., Wall, E.A., Yadava N., Heuck A.P., Ferrick, D.A., Henry R.R., McDonald W.G., Colca J.R., Simon M.I., Ciaraldi T.P., Murphy A.N. (2013) Thiazolidinediones are acute, specific inhibitors of the mitochondrial pyruvate carrier. Proc. Natl. Acad. Sci., 110(14):5422-7.
Compton C., Kim C., Griner N.B., Potluri P., Scheffler I.E., Sen S., Jerry D.J., Schneider S., Yadava N. (2011): Mitochondrial dysfunction impairs tumor suppressor p53 expression/function. J. Biol. Chem., 286(23): 20297-20312.
Gerencser A.A., Neilson A., Choi S.W., Edman U., Yadava N., Oh R.J., Ferrick D.A., Nicholls DG., Brand MD. (2009) Quantitative Microplate-Based Respirometry with Correction for Oxygen Diffusion. Anal Chem., 81: 6868-6878.
Chinta SJ., Rane A., Yadava N., Andersen JK., Nicholls DG., Polster, BM. (2009) Reactive oxygen species regulation by AIF- and complex I-depleted brain mitochondria Article: Free Rad. Biol. Med., 46(7):939-47.
Contact Info

Endocrine & Diabetes Division
Baystate Health/Tufts University School of Medicine
3601 Main Street, Room 2114
Springfield, MA 01107

(413) 794-0786