A 24 hour postfertilization zebrafish embryo was fixed and cleared. Slow twitch muscle fibers were labeled with anti-myosin heavy chain monoclonal antibody, F59 followed by TRITC conjugated secondary antibody.
The muscle sheet was imaged with a Nikon C1 scanning confocal system with a Nikon TE 2000 inverted microscope. Images were processed with the C1 EZ-Viewer software.
Department of Biology