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Center for Agriculture, Food, and the Environment

Integrating research and outreach education from UMass Amherst

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Jun Zhang

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Jun Zhang
Project Title: Deleting Nodule Specific Genes Using CRISPR-Cas9 to Understand Genes Involved in Symbiotic Nitrogen Fixation
Program Year: 2023
Major: Biochemistry and Molecular Biology
Supervisor: Dong Wang
Project description

This summer I was able to work in the Wang lab which focuses their research on the model legume Medicago truncatula. Legumes are important because they use these organs on their roots called nodules to fix atmospheric nitrogen into usable forms through the use of symbiotic nitrogen fixation. By studying the genes that are involved in symbiotic nitrogen fixation we can gain a better understanding of the pathways involved and the proteins that are necessary for functional nodule formation. This can be applied to agriculture as we can investigate alternative environmentally friendly ways of providing nitrogen to crops. We used CRISPR-Cas9 as a gene editing tool to knockout genes and study its effects. This was done by a two guide RNA construct that was designed to delete across a span of two nodule specific genes called nodulin 22 and nodulin 25.

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Deleting Nodule Specific Genes Using CRISPR-Cas9 to Understand Genes Involved in Symbiotic Nitrogen Fixation

Research

  • Mass Agricultural Experiment Station
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Center for Agriculture, Food, and the Environment

 

Stockbridge Hall,
80 Campus Center Way
University of Massachusetts Amherst
Amherst, MA 01003-9246
Phone: (413) 545-4800
Fax: (413) 545-6555
ag [at] cns [dot] umass [dot] edu (ag[at]cns[dot]umass[dot]edu)

 

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Nutrition Education

Value-Added Food

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