B-cell Protein Targets:

IgM: 1.14 available

Deluca, D., Wilson, M., and Warr, G.W. ((1983) Lymphocyte heterogeneity in the trout, Salmo gairdneri, defined with monoclonal antibodies to IgM. Eur J Immunol 13, 546-51.

IgT CH2: Protein produced; mice immunized August 2009; Fusion performed December 2009 (UMass Dartmouth); no clones recognizing native antigen. Trying new recombinant protein and Peptide immunizations.

IgT CH4: Protein produced; mice immunized August 2009; 3 fusions performed December 2009 (UMass Dartmouth); no clones recognizing native antigen. Trying new protein and Peptide immunizations.

IgL1: Peptide-conjugate produced; Successful product; Characterization underway.

IgL2: Peptide-conjugate produced; Successful product; Characterization underway.

IgL3: Peptide-conjugate produced; Successful product; Characterization underway.

IgD: Peptide-conjugate produced February 2010; Fusion scheduled April 2010(UMass Dartmouth)

secIgM: Peptide-conjugate produced February 2010; Immunizations scheduled April 2010 (UMass Dartmouth)

CD79a: Protein produced March 2010(USGS); Immunizations scheduled April 2010 (UMass Dartmouth)

CD79b: Protein produced March 2010(USGS); Immunizations scheduled April 2010 (UMass Dartmouth)

T-cell Protein Targets:

CD3: Protein produced; mice immunizaed September 2009-- March 2010; Fusion performed December 14th 2009 (UMass Dartmouth); No clones recognizing native antigen. Remaining mice rescreened, 1 sera positive on flow; fusion scheduled March 2010

CD4: Protein produced; mice immunized September 2009-- March 2010; Fusion performed December 21st, 2009 (UMass Dartmouth). No clones recognizing native antigen. Remaining mice rescreened, none sero-converted for native protein. Peptide approach is being explored in parallel to Recombinant protein

CD4REL: Protein produced; mice immunized September 2009-- March 2010; Fusion performed December 21st, 2009 (UMass Dartmouth). No clones recognizing native antigen. Remaining mice rescreened, none sero-converted for native protein. Peptide approach is being explored in parallel to Recombinant protein.

CTLA: Protein production scheduled Late 2010 (USGS).

TCRα: Protein production finished March 2010(Cornell); immunizations begin late March 2010.

TCRβ: Protein production finished March 2010(Cornell); immunizations begin late March 2010.

TCRγ: Protein production finished March 2010(Cornell); immunizations begin late March 2010.

CD28: Protein production scheduled June 2010 (USGS).

P56lck: Monoclonal antibody produced 2009 (USGS)

Dendritic cell Protein Targets:

CD83: Protein produced; mice immunized Spetember 2009; Mice have sero-converted to native protein; Fusion scheduled April 2010 (UMass Dartmouth).

Cytokines and Chemokines:

IFN1: Protein production scheduled May 2010 (USGS).

IFN2: Protein production scheduled May 2010 (USGS).

IFN3: Protein production scheduled May 2010 (USGS).

IFN4: Protein production scheduled May 2010 (USGS).