U.S. Veterinary Immune Reagent Network

Equine

Recombinant proteins have been made for the following genes: CCL2, CCL3, CCL5, CCL11, CXCL9, CXCL10, GM-CSF, IL-1β, IL-2, IL, 4, IL-6, IL-13, IL-15, IL-17A, IFN-α1

Recombinant proteins are available from Kingfisher Biotech, Inc.

Recombinant proteins will be made for the following genes: IL-10, IL-12p35, IL-12p40, IL-18, IL-23, TGFβ, TNF-α

Monoclonal antibodies developed by US-VIRN: Monoclonal antibody development for the horse is based on a priority list for equine reagents that was initially developed during a Havemeyer workshop at Cornell University in 2003. Since then, this list was adjusted according to needs expressed by the equine research community. A need for equine reagents can still be expressed by contacting the species coordinator.

Characterized monoclonal antibdies to equine cytokines and CD markers can be obtained from Cornell University. For these monoclonals, proof of specificity was obtained and is available in a published format. Click here for a list of the available monoclonal antibodies, confirmed applications, and links to the corresponding abstracts: http://courses2.cit.cornell.edu/eagnerlab/research/reagents/htm.

Monoclonal antibodies will be made to:

Cell Surface Molecules:

CD14: Completed; 8 mAbs, detection of native protein confirmed (flow cytometry, cell sorting, Western blotting) (Cornell). http://courses2.cit.cornell.edu/eagnerlab/research/reagents/htm.

CD23: Completed; 7 mAbs, detection of native protein confirmed (Cornell).

CD25: Protein expressed, fusion completed, characterization of mAbs ongoing(Cornell).

CD28: Completed; Fusion will be repeated (Cornell).

CD40: Completed; Fusion will be repeated (Cornell).

FcεRIα: Completed; Fusion completed, characterization of mAbs ongoing(Cornell).

TCRα: Protein purified; immunization ongoing(Cornell).

TCRγ: Protein purificiation; fusion planned(Cornell).

TCRδ: Protein purification; fusion planned(Cornell).

IgD: In expression vector; protein expression planned(Cornell).

Cytokines and Chemokines:

IL-4: Immunization with mammalian expressed protein completed; 1 mAb, detection of native protein confirmed (ELISA, flow cytometry, Western blotting, multiplex assay)(Cornell). http://courses2.cit.cornell.edu/eagnerlab/research/reagents/htm.

IL-2: Immunization with yeast protein completed; 1 mAb; detection of native protein confirmed (ELISA, flow cytometry, multiplex assay)(Cornell)

IL-10: Immunization with mammalian expressed protein completed; 3 mAbs; detection of native protein confirmed (ELISA, flow cytometry, multiplex assay) (Cornell) http://courses2.cit.cornell.edu/eagnerlab/research/reagents/htm.

IL-6: Immunization with yeast protein; fusion completed; several mAbs; characterization ongoing; detection of native IL-6 not yet confirmed (Cornell).

IL-1β: Immunization with yeast expressed protein; fusion completed; fusion will be repeated with protein expressed in mammalian cells(Cornell).

IL-5: Immunization with yeast expressed protein; fusion completed, characterization of mAbs ongoing; detection of native IL-5 not yet confirmed (Cornell).

IL-13: Immunization with yeast expressed protein stopped, needs repeat with protein expressed in mammalian cells (Cornell).

IL-17A: Immunization with yeast expressed protein; fusion completed, characterization of mAbs ongoing; ddetection of native IL-17A confirmed (Cornell).

GM-CSF: Immunization with yeast expressed protein; fusion completed; needs repeat with protein expressed in mammalian cells(Cornell).

CCL2: Immunization with yeast expressed protein; fusion completed; several mAbs; characterization ongoing; detection of native CCL2 confirmed for 7 mAbs(ELISA)(Cornell).

CCL3: Immunization with yeast expressed protein; fusion completed; 1 mAb; detection of native CCL3 confirmed (flow cytometry).

CCL5: Immunization with yeast expressed protein; fusion completed; 1 mAb; characterization ongoing; weak detection of native CCL5 (flow cytometry) (Cornell).

CCL11: Immunization with yeast expressed protein; fusion completed; several mAbs; characterization ongoing; detection of native CCL11 not yet confirmed (Cornell).

CXCL9: Immunization with yeast expressed protein; fusion completed; needs repeat with protein expressed in mammalian cells (Cornell).

CXCL10: Immunization with yeast expressed protein; fusion completed; needs repeat with protein expressed in mammalian cells(Cornell).

Click here for a list of equine reagents currently available: http://www.ca.uky.edu/Gluck/HorohovDW_EIR.asp The list represents on overview about reagents from multiple sources. Note that the specificity has not been confirmed for all the reagents on that list.

Click here to download the survey form.
Then fill it out, and send it back to the appropriate species coordinator.

Last updated: February 2012