Catfish

Primers
Bioassays
References for Genes
Publications & Posters
Commercially Available Reagents


Recombinant proteins will be produced for the following genes:

catfish Type I IFN, Type II IFN, soluble FcµRI (IpFcRI), IL-1, CXCL8


Monoclonal antibodies will be made to:

Cytokines and Chemokines:

IFNγ:Has been cloned and recombinant protein production is targeted.

IL-1:Gene sequence information has been sent to Kingfisher Biotech for protein production.

CXCL8:Protein produced by Kingfisher Biotech. Protein sent to LSU for bioassay. Monoclonals will be produced (UMiss Med Ctr, Jackson)


Cell Surface Molecules:

β2-microglobulin;: Protein produced; hybridoma screening and characterization ongoing (UMass Dartmouth and U Miss MS Med Ctr, Jackson). remaining mice have good response to native protein when measured on flow.

CD3 γ/δ: Protein produced and sent to UMass Dartmouth for mouse immunizations; mice not recognizing native protein by flow. Immunizations continue.

CD4: Protein produced; mice immunized; fusion re-targeted (U Mass Dartmouth); screening and characterization will be performed at U Miss MS Med Ctr, Jackson.

CD8α and CD8β: Protein production ongoing (U Miss MS Med Ctr, Jackson).

IgD: Protein produced; hybridoma screening confirms two stable clones (8F8 and 2C12). Contact Dr. Melanie Wilson for further information.

IgL λ: Protein produced; mice immunized; fusion re-targeted (UMass Dartmouth); screening and characterization will be performed at U Miss MS Med Ctr, Jackson.

IgLσ: Protein produced; 4 mAbs produced; contact Dr. Melanie Wilson (U Miss MS Med Ctr, Jackson) New fusion performed February 2010 to try to make IgG hybridomas; 5 clones positive by flow.

TCRα:Protein produced(Cornell); hybridoma production in process (UMiss Med Ctr, Jackson).

TCRγ:Protein produced(Cornell); hybridoma production in process (UMiss Med Ctr, Jackson).







Click here to download the survey form.
Then fill it out, and send it back to the appropriate species coordinator.



Last updated: February 2012



Dr. Melanie Wilson
Species Coordinator, Catfish





Dr. Eva Bengten
Species Coordinator, Catfish


Species Coordinators

Melanie Wilson
University of Mississippi
mwilson@microbio.umsmed.edu

Eva Bengten
University of Mississippi
ebengten@microbio.umsmed.edu


Catfish bioassays, genes, and recombinant proteins.



Species Collaborator

V. Gregory Chinchar, Ph.D.
University of Mississippi

Harry Dickerson, Ph.D.
University of Georgia

Larry Hanson, Ph.D.
Professor, Mississippi State

Liliana Jaso-Friedman, Ph.D.
University of Georgia

Mark Lawrence, D.V.M., Ph.D.
Mississippi State University

Sylvie Quiniou, D.V.M., Ph.D.
USDA-ARE CGRU

Charlie Rice, Ph.D.
Clemson University

Geoff Waldbeiser, Ph.D.
USDA-ARE CGRU, S


Species Consultants

Zhanjiang (John) Liu
Auburn University

Thomas McConnell
East Carolina University

Norman Miller
University of Mississippi

Phillip Kleisus
USDA-ARS (Alabama)

Gregory Warr
Medical University of South Carolina