CURRICULUM VITAE

 

 

Name:                         Barbara Anne Osborne

 

Home Address:          54 Depot Road, Leverett, MA  01054

 

Education:                  June 1970 ,  B.A.  English, Moravian College, Bethlehem, PA

                                    August 1979,  Ph. D. Genetics, Stanford University, Stanford, CA

 

Employment: 

            1970-1972       Elementary School Teacher, Lindenwold Public Schools

            1972-1974       Research Assistant, University of Maryland

            1974-1975       Microbiologist, National Institutes of Health

            1975-1977       Research Assistant, Stanford University

            1977-1979       Graduate Student, Stanford University

            1979-1981       Damon Runyon-Walter Winchell Postdoctoral Fellow

                                    National Institutes of Health

            1981-1983       Staff Fellow, National Institutes of Health

            1983-1985       Five Colleges Research Associate, Amherst College

            1985-1989       Assistant Professor

            1989-1995       Associate Professor

            1995-present    Professor

                                    Department of  Veterinary and Animal Sciences

                                    University of Massachusetts

                                    Amherst, Massachusetts

            1992                Visiting Associate Professor, Department of Biology, MIT

                                                Laboratory of Dr. Robert Horvitz

            1995                Visiting Scientist, Whitehead Institute, Cambridge, MA

                                                Laboratory of Dr. Robert Weinberg

            1999-2000       Visiting Professor, Whitehead Institute, Cambridge, MA

                                                Laboratory of Dr. Robert Weinberg

            1998-2005       Co-founder, Owner and Senior Consultant, Hematech, LLC

 

Honors:          

            1977-1979       USPHS Predoctoral Fellowship

            1979-1981       Damon Runyon-Walter Winchell Postdoctoral Fellowship

            1995                USPHS Senior Fellowship for sabbatical salary support

            1996 -              Member, Whitehead Institute Board of Associates

            1997                John Amos Comenius Alumni Award for Outstanding Achievement, Moravian College

1999-2000       Samuel Conti Award for excellence in research, Univ. of Mass.

2003                Chancellor’s Distinguished Lecturer, Univ. of Mass.

2005                Dean’s Award, NRE, Univ. of Mass.

2006                Chancellor’s Award for Outstanding Accomplishments in Research

Service:

           

            1987-1993       Member, NIH Immunology, Virology, Pathology Study Section

                                    Ad-hoc reviewer, NSF and USDA

            1993-present    Senior Editor, East Coast Office of Cell Death & Differentiation

            1993-present    Ad-hoc reviewer for NIH grants and site visits

1995-1999         Member, National Multiple Sclerosis Society grant review panel

            1998-2005       Editorial Board, Journal of Immunology

                                    2000-2005       Member, Damon Runyon-Walter Winchell Board of Scientific Advisors

                                    2000- 2005 Chair, Board of Scientific Counselors, NIAID, NIH

                                    2001- 2005      FASEB Finance Committee

            2001- 2004      AAI Finance Committee

 

 

Patents:  Expression of Xenogenous (Human) Immunoglobulins in Cloned Transgenic Ungulates.  US Patent # 09/988,115.

            Production of Ungulates, Preferably Bovines, That Produce Human   

            Immunoglobulins.  US Patent # 09/714,185

 

 

 

Current Grants:

 

NIH RO1 AI49361                Notch signaling in T cell function

10/01/03 – 9/30/08                              $345,835/year direct + indirect            

 

NIH RO1 AI057652              Polyclonal human antibody in cloned HAC transgenic cows

10/01/03 – 9/30/06                              $379,275/year direct +indirect

 

P01 AG025531-01                              Targeting Multiple Diseases Through g-Secretase

09/01/06-8/31/11                                             $1,025,674/year  direct + indirect

 

Publications:

 

1.                  Kos, K. A., B. A. Osborne and R. A. Goldsby. 1975.  Inhibition of group A arbovirus antigen production and replication in cells enucleated with cytochalsin B.  J.  Virol. 15:913.

 

2.                  Glode, L. M., I. Scher, B. A. Osborne and D. L. Rosenstreich. 1976.  Cellular mechanism of endotoxin unresponsiveness in C3H/HeJ mice.  J. Immunol. 116:454.

 

3.                  Herzenberg, L. A., S. J. Black, M. R. Loken, K. Okumura, W. van der Loo, B. A. Osborne, D. Hewgill, J. W. Goding, G. Gutman and N. Warner. 1976. Surface markers and functional relationships of cells involved in murine B cell differentiation.  In: Origins of Lymphocyte Diversity.  Proceedings of the Cold Spring Harbor Symposium On Quantitative Biology.  Vol.  XLI:33.

 

 

4.                  Warner, N. L., J. W. Goding, G. Gutman, G. W. Warr, L. A. Herzenberg, B. A. Osborne, W. van der Loo, S. J. Black and M. R. Loken. 1977.  Allotypes of mouse IgM immunoglobulin.  Nature 265:447.

 

5.                  Goldsby, R. A., B. A. Osborne, E. Simpson and L. A. Herzenberg. 1977.  Hybrid cell lines with T cell characteristics.  Nature 267:707. 

 

6.                  Goldsby, R. A., B. A. Osborne, D. M. Murphy, E. Simpson, J. Schroeder and L. A. Herzenberg.         1978. Somatic cell hybrids with T cell characteristics.  In: The Immune Response: Genetics and Regulation.  Vol.  VI:265.

 

7.                  Osborne, B. A., R. A. Goldsby and L. A. Herzenberg. 1978.  Selective expression of loci in the I-J region on T cell hybrids.   Current Topics in Microbiology and Immunology  81:217.

 

8.                  Goldsby, R. A., B. A. Osborne, D. Suri, A. Mandel, J. Williams, E. Gronowiez and L. A. Herzenberg. 1978.  Production of specific antibody without specific immunization.  Current Topics in Microbiology and Immunology  81:149.

 

9.                  Black, S. J., J. W. Goding, G. Gutman, L. A. Herzenberg, M. R. Loken, B. A. Osborne, W. van der Loo and N. L. Warner. 1978.  Immunoglobulin isoantigens (allotypes) in the mouse.  V. Characterization of IgM allotypes. Immunogenetics 7:213.

 

10.              Goldsby, R. A., B. A. Osborne, D. Suri, J. Williams and A. Mandel. 1978.  Preservation of the polyclonal response by hybridization: Specific antibody without specific immunization.  Current Microbiology 2:157.

 

11.              Goldsby, R. A., B. A. Osborne and E. G. Engleman. 1979.  Identification, isolation and characterization of a human T cell population by a monoclonal antibody. Current Microbiology 3:141.

 

 

12.              Engleman, E. G., C. Benike, B. A. Osborne and R. A. Goldsby. 1978.  Functional characteristics of human T cell subpopulations distinguished by monoclonal antibody.  Proc.  Nat.  Acad.  Sci. 77:1607.

 

13.              Suomalainen, H. A., R. A. Goldsby, B. A. Osborne and J. Schroder. 1980.  Mouse/human T cell hybrids resetting with sheep red blood cells.  Scan. Journal Immunol. 11: 163.

 

14.              Goldsby, R. A., B. A. Osborne and E. G. Engleman. 1980.  Characterization of a human T cell population identified and isolated by a monoclonal antibody.  In: Monoclonal Antibodies.  R. H. Kennett, T. J. McKean, K. B. Bechtol, eds. p. 121, Plenum Press.

 

15.              Metzger, J-J., G. Gilliland, J. K. Lunney, B. A. Osborne, S. Rudikoff and D. Sachs. 1981.  Transplantation in miniature swine.  IX.  Swine histocompatibility antigens: Isolation and purification of papain solubilized SLA antigens. J. Immunol 127:769.

 

16.              Singer, D. S., D. Camerini-Otero, L. Satz, B. A. Osborne, D. Sachs, and S. Rudikoff. 1982.  Characterization of a porcine genomic clone encoding a major transplantation antigen: expression in mouse L cells.  Proc.  Natl.  Acad.  Sci. 79:1403.

 

17.              Osborne, B. A., and S. Rudikoff. 1983.  Murine thymocyte and splenocyte la antigens are indistinguishable. J. Immunol. 131:1386.

 

 

18.              Roth, J. A., E. A. Grimm, B. A. Osborne, R. K. Gupka and R. S. Ames. 1982.  Immunoregulatory factors derived from human tumors.  II.  Purification and further biochemical characterization of factors that suppress lymphocyte proliferation responses.  J. Immunol. 130:303.

 

19.              Osborne, B. A., S. Rudikoff, J. Lunney and D. Sachs. 1983.  Two-dimensional gel analysis of gene products of the miniature swine SLA and la loci. J. Immunol. 131:2939.

 

20.              Lunney, J., B. A. Osborne, S. 0. Sharron, C. Devaux, M. Pierres and D. Sachs. 1983.  Sharing of la antigens between species.  IV.  Interspecies cross reactivity of monoclonal antibodies Directed against polymorphic mouse la determinants. J. Immunol. 130:2786.

 

 

21.              Osborne, B. A., T. E. Golde, R. L. Schwartz and S. Rudikoff. 1985.  Evolution of the mouse IgA gene: Nucleotide sequence comparison of IgA in Balb/c and Mus pahari. Current Topics in Immunology l27:141.

 

 

22.              Osborne, B. A., T. E. Golde, R. L. Schwartz and S. Rudikoff. 1988.  Evolution of the IgA heavy chain gene in the genus Mus.  Genetics 119:925.

 

23.              Ferguson, S.E, S. Rudikoff and B.A.Osborne. 1988.  Interaction and sequence diversity among T15 VH genes in CBA/J mice. J. Exp.  Med. 168:1339.

 

24.              Ferguson, S. E., M. P. Cancro, S. Rudikoff and B. A. Osborne. 1989.  T15 VH gene evolution in wild mice: The T15 VH family in two wild populations of Musculus domesticus, Centerville Light and Watkins Star.  J. Exp. Med. 170:1811.

 

25.              Osborne, B.A., S.E. Ferguson, S. Szabo and S. Sylvers. 1989.  Evolution of immunoglobulin genes.  In: Molecular Evolution.  M. Clegg and S. O'Brien, editors, p. 19-28, Wiley-Liss.

 

26.              Ravichandran, K.S., A.R. Semproni, R.A. Goldsby and B.A. Osborne. 1992.  Immunoglobulin V. usage analysis by fluorescent in situ hybridization and flow cytometry.  J.Immunol. Meth. 153:249-25

 

27.              Ravichandran, K.S., B.A. Osborne and R.A. Goldsby. 1993.  Quantitative analysis of the B cell repertoire by limiting dilution analysis and fluorescent in situ hybridization.  Cell. Immunol. 154:309-327.

 

28.              Schwartz, L.M., S.W. Smith, M.E.E. Jones and B.A. Osborne. 1993.  Do all programmed cell deaths occur via apoptosis?  Proc.  Natl.  Acad.  Sci. 90:980-984.

 

29.              Lowe, S., E.M. Schmitt, S.W. Smith, B.A. Osborne and T. Jacks. 1993. p53 is required for radiation-induced apoptosis in mouse thymocytes.  Nature 362:847-849.

 

30.              Schwartz, L.M. and B.A. Osborne. 1993. Programmed cell death, apoptosis and killer genes.  Immunol. Today 14:582-590.

 

31.              Punt, J.A., B.A. Osborne, Y. Takahama, S. Sharrow and A. Singer. 1994. Negative selection of  CD4+ CD8+ thymocytes by T cell receptor-induced apoptosis requires a costimulatory signal that can be provided by CD28. J. Exp. Med. 179:709-713.

 

32.              Liu, Z.-G, S.W. Smith , K. McLaughlin, L.M. Schwartz and B.A. Osborne. 1994. Apoptotic signals delivered through the T-cell receptor of a T-cell hybrid require the immediate-early gene nur77.  Nature 367:281-284.

 

33.              Schwartz, L.M. and B.A. Osborne. 1994. Ced-3/Ice: Evolutionarily conserved regulation of cell death.  BioEssays 16:387-390.

 

34.              Smith, S.W. and B. A. Osborne.  1995.  Molecular events in thymocyte apoptosis. In: Current Topics in Micro. & Immunol, Vol. 200:147-162

 

35.              Osborne, B.A. 1995. Induction of genes during apoptosis; Examples from the immune system. Seminars in Cancer Biology 6:27-34.

 

36.              Schwartz, L.M. and B.A. Osborne, eds.  1995. Methods in Cell Biology: Cell Death, Vol. 46; Academic Press, San Diego, CA.

 

37.              Osborne, B.A. and L. M. Schwartz. 1994. Genes that regulate apoptosis. Trends in Cell Biol. 4:394-399.

 

38.              Osborne, B.A., S.W. Smith, Z.-G Liu, K. McLaughlin and L. M. Schwartz. 1995. Transient transfection assays to examine the requirement of putative cell death genes.  In: Methods in Cell Biology: Cell Death, Vol. 46:99-106, Academic Press, San Diego, CA.

 

39.              Osborne, B.A. 1994.  Intracellular regulators of death. Cell Death & Differentiation 2:69.

 

40.              Osborne, B. A., S.W. Smith, Z.-G. Liu, K. McLaughlin, L. Grimm and L. M. Schwartz. 1994.Identification of genes induced during apoptosis in T lymphocytes. Immunological Reviews, Vol. 141, 301-320.

 

41.              McLaughlin, K.A., S. W. Smith, C.E. Milligan, W. Bielke, L. M. Schwartz and B.A. Osborne. 1995.  The isolation and characterization of cell death genes. In: Handbook of Experimental Immunology, Blackwell Scientific Press, Vol.4.

 

42.              Parng, C.-L., S. Hansal, R.A. Goldsby and B. A. Osborne. 1995.  Gene conversion generates immunoglobulin gene diversity in cattle. Ann. New York Acad. Sci., 764:155-157.

 

43.              Osborne, B. A. And L. M. Schwartz.  1995.  Cell death suffers a TKO.  BioEssasys 17:557-559.

 

44.              Parng, C.-L, S. Hansal, R.A. Goldsby and B.A. Osborne. 1996.  Gene conversion generates immunoglobulin gene diversity in cattle.  J. Immunol. 157:5478-5486.

 

45.              McLaughlin, K., B. A. Osborne and R. A. Goldsby. 1996. The role of oxygen in thymocyte apoptosis. Eur. J. Immunol., 26:1170-1175.

 

 

46.              Meyer, A., C. -L. Parng, S. Hansal,  B.A. Osborne and R. A. Goldsby. 1996.  Immunoglobulin gene diversification in cattle.  International Review of Immunology, 15:165‑183.

 

47.              Osborne, B. A., S. W. Smith, K.A. McLaughlin, L. Grimm, T. Kallinch, Z-G. Liu and L. M. Schwartz.  1996.  Genes that regulate apoptosis in the mouse thymus.  Journal of Cellular Biochemistry, 60:18-22.

 

48.              Grimm, L. A., Goldberg, A. L., Poirier, G.G., Schwartz, L.M. and B.A. Osborne. 1996. Proteasomes play an essential role in thymocyte apoptosis. EMBO J., 15:3835-3844.

 

49.              Osborne, B. A.  1996. Apoptosis and the maintenance of homeostasis in the immune system. Current Opinion in Immunology, 8:245-254.

 

50.              Osborne, B.A., Smith, S.W., McLaughlin, K.A., Grimm, L., Morgan, G.M. and R.A. Goldsby.  1996.  Genetic regulation of apoptosis in the mouse thymus. In Advances in Experimental Medicine and Biology. Vol. 406:199-208.

 

51.              Osborne, B. A.  1996. Cell death in Vertebrates: Lessons from the worm.  Trends in Genetics, 12:489-491.

 

52.              Jehn, B. and B.A. Osborne. 1996. Gene Regulation Associated with Apoptosis.  Critical Reviews in Biochemistry, 7:179-193

 

 

53.              Osborne, B. A. 1996. Keeping Ahead of the Reaper.  Cell Death and Differentiation, 3:445.

 

54.              Smith., S.W. and B.A. Osborne.  1997.  Private pathways to a common death.  J. of NIH Res. 9:33-37.     

 

 

55.              Grimm, L.M. and B.A. Osborne. 1998.  Apoptosis and the Proteasomes.  In Apoptosis: Biology and Mechanisms. pg 209-223, S. Kumar, editor, Springer, Inc.

 

56.              Lucier, M., Thompson, R., Osborne, B.A. and Goldsby, R.A.  1998.  Ig diversification mechanisms in cattle. J. Immunol. 61:5438‑44.

 

57.              Jehn, B., Bielke, W., Pear, W. and B.A. Osborne. 1999. Nur77 and Notch-1 cooperate in the regulation of cell death.  Cutting Edge/J. Immunol. 162:635-38

 

58.              Amsen, D., Osborne, B.A. and A.M. Kruisbeek. 1999. Costimulatory signals re required for induction of Nur77 protein during negative selection of CD4+, CD8+ thymocytes.  Proc. Natl. Acad. Sci., 96: 622‑627

 

59.              Osborne, B.A.  1999. Apoptotic signaling pathways in lymphocytes. In Cell Death, R. Lockshin, editor, John Wiley & Sons, Inc., in press. 

 

60.              Morgan, G., Smith S.W., Pak, J., Marshak-Rothstein, A., Fissore, R. and B. A. Osborne. 1999. Characterization of a mutant T cell line with defects in TCR-mediated apoptosis. Cell Death & Differen. 6:36-47.

 

61.             Grimm, L.M. and B.A. Osborne. 1999.  Apoptosis and the proteasome..  Results Probl Cell Differ. 23:209-28.

 

62.              Miele, L. and B.A. Osborne. 1999. Arbiter of differentiation and death: Notch signaling meets apoptosis.  J Cell Physiol. 181(3):393-409.

 

63.              Osborne, B.A. and L. Miele. 1999  Notch and the immune system. Immunity 11:653-663.

 

64.              Goldsby, R.A., Kindt, T.J. and B.A. Osborne. 1999.  Kuby Immunology, WH Freeman, NY, NY.

 

65.              Jeong Y., Osborne B.A. and R.A. Goldsby R.A. 2001.  Early Vlambda diversification in sheep.   Immunology.  103:26-34.

 

66.              Osborne BA. 2000.  Transcriptional control of T cell development.. Curr Opin Immunol. 12:301-6.

 

67.              Collas,P., Kline, R., Landsverk H.B., Hein,W.R.,Goldsby, R.A., Osborne, B.A. and T. Landsverk. 2000. Intercellular transfer of DNA in membrane-bounded 50-nanometer particles in bovine IPP. Eur J Cell Biol. 8:69-76.

 

68.               Cheng, P., Zlobin,A., Volgina V., Gottipati, S., Osborne, B.A., Miele, L., Gabrilovich, D.I.. 2001

69.              Notch-1 regulates NF-kappa B activity in hematopoietic progenitor cells. J Immunol. 167:4458-67.

 

70.              Palaga T, Osborne B. 2002. The 3D's of apoptosis: death, degradation and DIAPs. Nat Cell Biol. 4:E149-51.

 

71.              Kuroiwa Y, Kasinathan P, Choi YJ, Naeem R, Tomizuka K, Sullivan EJ, Knott JG, Duteau A, Goldsby RA, Osborne BA, Ishida I, Robl JM. 2002.  Cloned transchromosomic calves producing human immunoglobulin. Nat Biotechnol. 20(9):889-94.

 

72.              Weijzen, S,.Braid, E, Mehta, R,  Jonkheer,S,  Zlobin,A.,  Osborne,BA,  Gottipati  S., Aster, J.,  Hahn, WC, Kast, M. Miele, L., 2002. Activation of Notch-1 signalling maintains the neoplastic phenotype in Ras-transformed cells. Nature Medicine. 8:979-986.

 

73.              Lee, JM, Lee KH, Weidner M, Osborne BA and SD Hayward. 2002. Epstein-Barr virus EBNA-2 blocks Nur77-mediated apoptosis. Proc. Natl. Acad. Sci., 99:11878-11883.

 

74.              Ganguly BJ, Tonomura N, Benson RM, Osborne BA, Granowitz EV. 2002.  Hyperbaric oxygen enhances apoptosis in hematopoietic cells. Apoptosis. 7:499-510.

 

75.              Palaga, T., Leiber., T., Young, M., Cumberledge, S and Osborne, B.A. (2003) Notch/Su(H) regulated Drosophila cell death through interactions with the ecdysone receptor pathway. (Submitted)

 

76.              Tonomura, N., McLaughlin, K., Grimm, LM, Goldsby, RA and BA Osborne. 2003. Glucocorticoid-induced apoptosis of thymocytes:Requirement of proteasome-dependent mitochondrial activity.  J. Immunol, 170(5):2469-78.

 

77.              Goldsby, R.A., Kindt, T.J., Osborne, B.A. and J. Kuby. 2002. Immunology, 5th ed., WH Freeman, NY, NY

 

78.              Palaga, T., Miele, L, Golde, TE and BA Osborne.  2003. Notch-1 is required for the proliferation of peripheral T cells and IFNg production in CD8 T cells. J. Immunol. 171:3019-3024.

 

79.                 Minter, L. M. and B.A. Osborne (2003) Cell death in the thymus-it’s all a matter of contacts.  Seminars in Immunol. 15:135-144.

 

80.                 Cheng, P. , Nefedova, Y, Miele, L., Osborne, B.A. and Gabrilovich, D. 2003. Notch signaling is necessary but not sufficient for differentiation of dendritic cells. Blood. 102:3980-8.

 

81.              Nickoloff BJ, Osborne BA, and L.  Miele. 2003. Notch signaling as a therapeutic target in cancer: a new approach to the development of cell fate modifying agents. Oncogene. 22:6598-608.

 

82.              Benson RM, Minter LM, Osborne BA, and EV Granowitz.  2003. Hyperbaric oxygen inhibits stimulus-induced proinflammatory cytokine synthesis by human blood-derived monocyte-macrophages.  Clin Exp Immunol. 134:57-62.

 

83.              Laws, A. and B.A. Osborne.  2004. p53 regulates thymic Notch1 activation.
Eur J Immunol. 34: 726-34.

 

84.              Tonomura,N.,  Goldsby, RA., Granowitz, EV. and B. A.  Osborne.  2004.  Oxidative Stress and Thymocyte Apoptosis.  In Molecular Mechanisms of Programmed Cell Death, Kluwer Academic, NY, NY.

 

85.              Minter, L. M.,, D. M. Turley, P. Das, H. M. Shin, I. Joshi,  R. G. Lawlor, O. H. Cho, T. Palaga, S. Gottipati, J. C. Telfer, L. Kostura, A. H. Fauq, K. Simpson, K. A. Such, L. Miele, T. E. Golde, S. D. Miller and B. A. Osborne. 2005.  g-secretase inhibitors block in vivo and in vitro Th1 polarization by preventing Notch upregulation of t-bet.  Nature Immunology, 6:680-8.

 

86.              Granowitz EV, Tonomura N, Benson RM, Katz DM, Band V, Makari-Judson GP, Osborne BA.  2005. Hyperbaric oxygen inhibits benign and malignant human mammary epithelial cell proliferation.  Anticancer Res. 25:3833-42.

 

87.              Shin, HM, Minter LM, Cho OH, Gottipati, S, Fauq AH, Golde TE, Sonenshein GE and BA Osborne  2005. Notch1 Augments NF-kB Activity by Facilitating its Nuclear Retention. EMBO J.  25:129-38.

 

88.              Gottipati, S., Palaga T, Fortner KA, Rincón M., May MJ,  Ghosh S, Capobianco AJ, and BA. Osborne. 2006.  Notch-1 Regulates IFN-g Secretion Through Activation of NF-kB. submitted

 

89.              Morgan, GM,  Q. Guo, SL Chan, DS Gary, BA Osborne and  MP Mattson.  2006. Defects of immune regulation in the presenilin-1 mutant knock-in mouse. In press, April 2006, Neuromolecular Medicine

 

90.              Miele, LM, Golde TE and BA Osborne. 2006.  Notch signaling in cancer.  Current Molecular Medicine.  In Press.

 

91.              Osborne, BA and LM Minter. 2006. Notch signalin