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BD LSR II

The LSR II at the University of Massachusetts, Amherst is configured with 488 nm and 633 nm fixed alignment lasers. The primary blue 488 nm laser detects 5 fluorochromes while the red 633 nm laser detects 3 fluorochromes

Optical configuration

488 nm LASER

Detector	A	B	C	D	E	F	G
Fluorochrome	PE-Cy7	PerCP-Cy5.5	PE-Texas Red	PE, PI	FITC, GFP	SSC
Long pass filter	735	685	595	550	505	x	x
Band pass filter	780/60	695/40	610/20	575/26	530/30	488/10	x

633 nm LASER

Detector	A	B	C
Fluorochrome	APC-Cy7	Alexa 700	APC
Long pass filter	735	690	x
Band pass filter	780/60	710/50	660/20

Baseline PMT

Parameter	Voltage
FITC	600
PE	550
PE-Texas Red	550
PerCP-Cy5.5	800
PE-Cy7	750
APC	550
Alexa 700	500
APC-Cy7	700