| Seymour | Harry Lacoste |
| Audrey | Marcy Secora |
| Mushnik | Evan Kelly |
| Orin | Gordon Bedford |
| Ronnette | Heidi Crawley |
| Crystal | Kim Shaker |
| Chiffon | Nicole Gageres |
| Audrey 2 | Dolores Brown |
| Dream Audrey | Leslie Nye |
| Dream Children: | Elizabeth Bean |
| Adrena Garabedian | |
| Denton | Brian Girard |
| Bernstein | Tiago Rodrigues |
| Mrs. Luce | Carla Brunning |
| Skip Snip | JR Closs |
| Patrick | Scott Hamill |
| Ensemble: | Sara Collins |
| Aaron Fiske | |
| Tom Jaquith | |
| Justine Lore | |
| Kristi Sullo | |