Genomics Resource Lab
Automated DNA Sequencing -
Sample Submission
General recommendations
- Prepare your templates (see Template section).
- Prepare your primes (see Primers section).
- For none pre-mixed template and primer: please submit 10µL DNA template and 10µL primer in separate tubes. Do not mix template and primer together. Sample names must be written on the tubes.
- For premixed Reactions, please mix
5µL of DNA template at a concentration of 0.3µg/µL (Plasmid DNA), 20 ng DNA per 100 base pairs product/µL (PCR product), 0.4 µg/µL (Cosmids or lambda) or 0.1 µg/µL (Single-stranded phage) with
5µL of primer at a concentration of 5 µM.
Minimum final volume will be 10µL in 96 well, "V" bottom plates.
- For Ready-to-Run Plates, please use ABI MicroAmp Optical 96-Well Reaction Plate, Cat. No. N8010560.
- Users must include a printed Sequencing Order Form. The Sequencing Order Form is available here. If you have more than 16 samples, please also email your Sequencing Order Form to genomics@research.umass.edu.
- You may send or mail samples to:
> Genomics Research Lab
> University of Massachusetts
> Fernald Hall, B1
> 270 Stockbridge Road
> Amherst, MA 01003
> 413-545-1039
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