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Robert Bernatzky Associate Professor of
Plant Genetics & Breeding Education B.A. (Biology), Long
Island University, 1976 Research Interests Modern methods of plant
breeding Research Summary Molecular Markers For Plant Breeding And Genetics Recent advances in molecular biology have allowed the development of a new set of markers for plant chromosomes based on DNA sequences. Differences in length of DNA fragments generated by restriction enzymes can be used to mark specific chromosome positions. Linkage relationships of these markers can then be determined and chromosome maps can be constructed. If markers are found that are linked to important agronomic characters then these markers can be used to efficiently select for those characters in breeding programs. Molecular markers also provide information on chromosome organization and evolution, such as changes in copy number and positions of genes. Current research crops include species of Lycopersicon (tomato and its wild relatives), and American and Chinese chestnut (Castanea). Self-Iincompatibility In Plants Many plant families
express a system of self-incompatibility (SI) in which plants are
unable to self-fertilize. The genetics of these systems have been
studied intensively but have only recently been explored on the
molecular level. A better understanding of this important
reproductive strategy of plants could lead to more effective control
of fertilization. The family Solanaceae contains many genera with
simple SI systems. Most of the wild species of tomato are SI
although there are a few species and accessions that are
self-compatible (SC). Molecular cloning techniques are being used to
identify and characterize the components of SI and SC in tomato
species. Research Publications Weiner, J.L. and R. Bernatzky. 2001. Isolation of genes on chromosome 3 related to self-incompatibility in tomato through marker assisted selection and differential display, in preparation. Weiner, J.L. and R. Bernatzky. 2000. Effects of segments of chromosome 1 and 3 from L. hirsutum (LA1777) on anther and style length in L. esculentum background. Report of the Tomato Genetics Cooperative 50:48. Mangan, F. A. Carter, M. Mazzola, M. Rulevich, R. Hazzard, R. Bernatzky, M. Pearson, A. Smith, J.Baranek, P. Harmsen, L. Dow, M. Anderson, L. Colangione, D. Webber, P. Belanger, C. Touchette, and P. Fischer. 2000. The Introduction of Latino Crops to Farmers in the State of Massachusetts, USA. Proceedings of the Interamerican Society for Tropical Horticulture.391-393. McClure, B., B. Mou, S. Canevascini, R. Bernatzky. 1999. A small asparagine-rich protein required for S-allele-specific pollen rejection in Nicotiana. Proc. Natl. Acad. Sci. U.S.A. 96:13548-13553. Mangan, F, R. Hazzard, J. Howell, R. Wick, R. Bonanno, C. Hollingsworth, A.K. Carter, D. Ferro, A. Miller, R. Bernatzky, and S. Herbert. 1999. Integrated Pest Management of Peppers (Capsicum sp.) in the state of Massachusetts, USA (in Spanish). Proceedings of the InterAmerican Society for Tropical Horticulture 41:1-6. Chawla, B, R. Bernatzky, W. Liang and M. Marcotrigiano. 1997. Breakdown of self-incompatibility in tetraploid Lycopersicon peruvianum: Inheritance and expression of S-related proteins, Theoretical and Applied Genetics 95:992-996. Kubisiak, T.L., F.V. Hebard, C.D. Nelson, J. Jhang, R. Bernatzky, H. Huang, S.L. Agnagnostakis, and R. L. Doudrick. 1997. Mapping resistance to blight in an interspecific cross in the genus Castanea using morphological, isozyme, RFLP and RAPD markers. Phytopathology 87:751-759. Woeste, K., G. McGranahan and R. Bernatzky. 1996. The identification and characterization of a genetic marker linked to hypersensitivity to the cherry leaf roll virus in walnut. Molecular Breeding 2:261-266. Woeste, K., G. McGranahan and R. Bernatzky. 1996. Randomly amplified polymorphic DNA loci from a walnut backcross (Junglans hindsii X J. regia) X J. regia, Journal of the American Society for Horticultural Science 121:358-361. Bernatzky, R., R.H. Glaven, and B. Chawla. 1996. Recovery of S-locus homozygotes through wide hybridization in L. peruvianum. Report of the Tomato Genetics Cooperative. Bernatzky, R., R.H. Glaven and B.A. Rivers. 1995. S-related protein can be recombined with self-compatibility in interspecific derivatives of Lycopersicon. Biochemical Genetics 33:215-225. Marcotrigiano, M and R. Bernatzky. 1995. Arrangement of cell layers in the shoot apical meristems of periclinal chimeras influences cell fate. The Plant Journal 7:193-202. Bernatzky, R., Y. Zhang, G.R. Hackett and R.H. Glaven. 1995. Identification of the self-incompatibility progenitor alleles of the self-compatible mutation in Lycopersicon peruvianum. Report of the Tomato Genetics Cooperative 45:13. Bernatzky, R., R.H. Glaven and B.A. Rivers. 1994. S-related proteins are not sufficient to elicit a self-incompatible response in interspecific derivatives of Lycopersicon. In: Current Topics in Plant Physiology: Pollen-Pistil Interactions and Pollen Tube Growth, edited by A.G. Stephenson and T-h Kao, The American Society of Plant Physiologists, p. 279-280. Bernatzky, R. and D. D. Miller. 1994. Self-incompatibility is codominant in intraspecific hybrids of self-compatible and self-incompatible Lycopersicon peruvianum and L. hirsutum based on protein and DNA marker analysis. Sexual Plant Reproduction 7:297-302. Liang, W., Rivers, B.A. and R. Bernatzky. 1994. Molecular cloning of a second S-allele from Lycopersicon peruvianum and assessment of allele frequencies in populations based on DNA hybridization. Report of the Tomato Genetics Cooperative 44:22-23. Rivers, B.A. and R. Bernatzky. 1994. Protein expression of a self-compatible allele from Lycopersicon peruvianum: Introgression and behavior in a self-incompatible background. Sexual Plant Reproduction 7:357-362. Bernatzky, R. 1993. Genetic mapping and protein product diversity of the self-incompatibility locus in wild tomato (Lycopersicon peruvianum). Biochemical Genetics 31:173-184. Rivers, B.A., R. Bernatzky, S.J. Robinson and W. Jahnen-Dechent. 1993. Molecular diversity at the self-incompatibility locus is a salient feature in natural populations of wild tomato (Lycopersicon peruvianum). Molecular and General Genetics 238:419-427. Bernatzky, R. and D.L. Mulcahy. 1992. Marker-aided selection in a backcross breeding program for resistance to chestnut blight in the American chestnut. Canadian Journal of Forest Research 22:1031-1035. Mulcahy, D.L. and R. Bernatzky. 1992. Speeding restoration of the American chestnut by using genetic markers in a backcrossing program: An homage to Dr. Charles Burnham. Journal of the American Chestnut Foundation 7:33-36. Bernatzky, R, S-L Mau and A.E. Clarke. 1989. A nuclear sequence associated with self-incompatibility in Nicotiana alata has homology with mitochondrial DNA. Theor. Appl. Genet. 77:320-324. Anderson, M.A., G.I. McFadden, R. Bernatzky, A. Atkinson, T. Orpin, H. Dedman, G. Tregear, R. Fernley, and A.E. Clarke. 1989. Sequence variability of three alleles of the self-incompatibility gene of Nicotiana alata. Plant Cell 1:483-491. Ebert P.R., M.A. Anderson, R. Bernatzky, M. Altschuler, and A.E. Clarke. 1989. Genetic polymorphism of self-incompatibility in flowering plants. Cell 56:255-262. Bernatzky, R. and S.D. Tanksley. 1989. Restriction fragments as molecular markers for germplasm evaluation and utilisation. In: The Use of Plant Genetic Resources, edited by A.H.D. Brown, O.H. Frankel, D.R. Marshall, J.T. Williams, Cambridge University Press, Cambridge, UK. Bernatzky R., M.A. Anderson, and A.E. Clarke. 1988. Molecular genetics of self-incompatibility in flowering plants. Dev. Genet. 9:1-12. Tanksley S.D., R. Bernatzky, N.L. Lapitan, and J.P. Prince. 1988. Conservation of gene repertoire but not gene order in pepper and tomato. Proc. Natl. Acad. Sci. U.S.A. 85:6419-6423. Bernatzky, R. 1988. Restriction fragment length polymorphisms. In: Plant Molecular Biology Manual, edited by S.B. Gelvin, R.A. Schilperoort, D.P.S. Verma, Martinus Nijhoff, Dordrecht, Netherlands. Clarke, A.E., M.A. Anderson, R. Bernatzky, E.C. Cornish and S-L. Mau. 1988. Molecular aspects of self-incompatibility In: Molecular Basis of Plant Development, UCLA Symposia, Steamboat Springs, Colorado, USA. Bernatzky, R., E. Pichersky, V.S. Malik and S.D. Tanksley. 1988. CR1 -- A dispersed repeated element associated with the Cab-1 locus in tomato. Plant Mol. Biol. 10:423-433. Pichersky, E., N.E. Hoffman, V.S. Malik, R. Bernatzky, S.D. Tanksley, L.Szabo and A.R. Cashmore. 1987. The tomato Cab-4 and Cab-5 genes encode a second type of CAB polypeptide localized in Photosystem II. Plant Mol. Biol. 9:109-120. Tanksley, S.D., and R. Bernatzky. 1987. Molecular markers for the nuclear genome of tomato. In: Tomato Biotechnology, edited by D.J. Nevins and R.A. Jones, Alan R. Liss, New York. Pichersky, E., R. Bernatzky, S.D. Tanksley, V. Malik and A.R. Cashmore. 1987. Genomic organization and evolution of the RBCS and CAB gene families in tomato and other higher plants. In: Tomato Biotechnology, edited by D.J. Nevins and R.A. Jones, Alan R. Liss, New York. Bernatzky, R. and S.D. Tanksley. 1986. Toward a saturated linkage map in tomato based on isozymes and random cDNA sequences. Genetics 112:887-898. Pichersky E., R. Bernatzky, S.D. Tanksley, and A.R. Cashmore. 1986. Evidence for selection as a mechanism in the concerted evolution of Lycopersicon esculentum (tomato) genes encoding the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase. Proc. Natl. Acad. Sci. U.S.A. 83:3880-3884. Bernatzky, R. and S.D. Tanksley. 1986. Genetics of actin-related sequences in tomato. Theor. Appl. Genet. 72:314-321. Bernatzky, R. and S.D. Tanksley. 1986. Majority of random cDNA clones correspond to single loci in the tomato genome. Mol. Gen. Genet. 203:8-14. Bernatzky, R. and S.D. Tanksley. 1986. Using molecular markers to analyze genome organization and evolution. In: Plant Tissue and Cell Culture, edited by C.E. Green, D.A. Somers, W.P. Hackett and D.D. Biesboer, Alan R. Liss, New York. Vallejos, C.E., S.D. Tanksley, R. Bernatzky. 1985. Localization in the tomato genome of DNA restriction fragments containing sequences homologous to the rRNA (45S), the major chlorophyll a/b binding polypeptide and the ribulose bisphosphate carboxylase genes. Genetics 112:93-105. Bernatzky, R. and S.D. Tanksley. 1985. Chromosomal localization of nuclear sequences homologous to random cDNA clones in tomato. In: Plant Genetics, edited by M. Freeling, Alan R. Liss, New York. Pichersky, E., R. Bernatzky, S.D. Tanksley, R.W. Briedenbach, A.P. Kauch and A.R. Cashmore. 1985. Molecular characterization and genetic mapping of two clusters of genes encoding chlorophyll a/b binding proteins in Lycopersicon esculentum (tomato). Gene 40:247-248. Recent Abstracts Weiner, J.L., R. Bernatzky. 2001. Marker-assisted selection for genes affecting reproductive barriers in tomato (Lycopersicon esculentum Mill.). HortScience, in press. Dukovski, D., S. Hawkins, R. Bernatzky, and S. Han. 2001. Ethylene induction of flowering in bromeliads. HortScience, in press. Bernatzky, R., J. Benton, J. Weiner, A. Idnurm, T. Kubisiak, F. Hebard, and P. Sisco. 2000. Applications of genetic markers (RFLPs) to Castanea dentata and C. mollissima. Proceedings of the Northeast and Southwide Forest Disease Workshops, May 31-June 2, 2000, Shepherdstown, WV. Borden, M., R. Bernatzky, and R. Manley. 1999. Changes in freezing tolerance, abscisic acid concentration, and gene expression during cold acclimation of Acer rubrum fine roots. HortScience 34:208. Holm, D., and R Bernatzky. 1998. Breeding early blight resistance for tomato in Massachusetts. HortScience. 33:206. Li, Y.-L., R. Bernatzky, S.S. Han. 1998. Ethylene induction of flowering and gel analysis of apex proteins in Dutch iris 'Telstar'. HortScience 33:47. Technical Publications Hollingsworth, C., R. Hazzard, R. Bernatzky, and D. Ferro. 2000. Issues about genetically engineered vegetable crops. University of Massachusetts Extension Publication, 7 pp. Bernatzky, R., and T. MeKechnie. 2000. Restoring the American chestnut. University of Massachusetts Agriculture Experiment Station Publication, Accents 4:6. Mangan, F., A. Carter, R. Bernatzky. 1999. Latino crops for Massachusetts. University of Massachusetts Vegetable Notes, February, p. 3-4. Bernatzky, R. 1998. Saving and preserving tomato seeds. University of Massachusetts Extension Vegetable IPM Message 15: 3-4. Bernatzky, R. and A. Schilling. 1992. Methods for Southern blotting and hybridization. In: Plant Genomes: Methods for Genetic and Physical Mapping, edited by T.C. Osborn and J.S. Beckmann, Kluwer Academic Publishers. Bernatzky, R. 1988. Restriction fragment length polymorphisms. In: Plant Molecular Biology Manual, edited by S.B. Gelvin, R.A. Schilperoort, D.P.S. Verma, Martinus Nijhoff, Dordrecht, Netherlands. Bernatzky, R. and S.D.
Tanksley. 1986. Methods for detecting single or low copy sequences
in tomato on Southern blots. Plant Mol. Biol. Rep. 4:37-41. |
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