Global Protein Structure Issues
How are 3D macromolecular structures obtained?
- Accurate: 83% by X-ray crystallography, 15% by NMR
- Rough: Homology modeling, but templates available for
only ~20% of cases.
- Ab initio theory does not predict tertiary structure
accurately enough for most purposes.
What percentage of the human proteome has known structure?
- ~1% accurately (empirical).
- ~10% roughly (homology modeling).
- Solution: Structural Genomics?
(Not in the next few years.)
Some proteins are intrinsically unstructured to support their functions.
- ~10% of proteins are predicted to be fully disordered.
Examples (bound to structured proteins):
- ~40% of eukaryotic proteins have at least one long (>50 residues)
Eric Martz, University of Massachusetts, July 2003 (revised February 2004)