Equipment and Resources
The Muscle Biology and Imaging Lab is housed in a 1,900 sq ft facility with separate areas for muscle imaging, biochemistry procedures, muscle sectioning, and gel electrophoresis. The lab also includes separate space for subject muscle function testing and a fume hood and wet lab for biochemistry procedures.
The lab is equipped with a MICROM HM 505E Cryostat, a Nikon Eclipse E400 phase contrast fluorescent microscope (Spot Insight QE camera and EclipseNET software), EC135-90 power supply with gel electrophoresis and transfer systems, 2 spectrophotometers (UV and visible wavelengths), microplate photometers, waterbaths, distillers, refrigerated centrifuges, and an ultrasonic cell disruptor.
The lab has recently added equipment to analyze the molecular processes of muscle hypertrophy and atrophy using RNA and protein isolation. This equipment includes a laminar flow PCR workstation, Isotemp 145D digital dry-bath incubator, ND-1000 UV-Vis spectrophotometer, dual light transilluminator, hybridization incubator, and Phoretix TotalLab gel analysis software.
The Muscle Biology and Imaging Lab is based on the campus of UMass Amherst, one of the nation's top public research universities. The campus attracts over $140 million in externally sponsored research each year. Additional research services, equipment and facilities are available.