John M. Clark
I am a pesticide toxicologist with 40+ years’ professional experience and expertise in the fields of human exposure to pesticides, pesticide mode of action and molecular elucidation of resistance mechanisms. I have extensive experience studying the modes of action of pyrethroid, organophosphorus, carbamate, organochlorine, phenylpyrazole and macrocyclic lactone insecticides.
My research group has studied the neurotoxicology of insecticides, primarily dealing with Ca2+-dependent neurotransmitter release and voltage sensitive Ca2+-channels, in rat brain tissues for some 25+ years using a variety of biochemical approaches and techniques including: agonists/antagonists, radioisotopes, fluorescent probes, electrophysiology and functional and reverse genetics. We have studied the mechanisms of insecticide resistance in a variety of blood-sucking insect that vector disease and have used functional genomics to develop DNA-based diagnostics to monitor the evolution of resistance in vector populations.
My research group, along with collaborators, are studying: (1) the role of insecticides in the increase occurrence of childhood obesity and type 2 diabetes; (2) the mode of action/resistance of a number of new insecticides for the control of human ectoparasites (e.g., lice, bed bugs and mosquitoes) and the development of sustainable resistance management strategies; (3) the effect of neurotoxicants on native channels and receptors expressed in neurolemma fragments injected into Xenopus laevis oocytes as a high-throughput screen to identify novel target sites; (4) the role of the innate immune response in the evolution of vector competence in human lice and (5) PFAS disruption of receptor-mediated endocytosis and later life metabolic dysfunction.
Learn more at www.vasci.umass.edu/research-faculty/john-marshall-clark
- BS Zoology, Univ. of Wisconsin-Madison, 1972
- MS Entomology (Insecticide Toxicology), Univ. of Wisconsin-Madison, 1977
- PhD Toxicology, Michigan State University, 1981