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Igor A. Kaltashov

Professor

Bioanalytical chemistry; biological and medicinal applications of mass spectrometry; structure, properties, delivery and mechanism of action of protein-based therapeutics.

Current Research
Biopharmaceuticals are a unique class of medicines due to their extreme structural complexity. The structure of these therapeutic proteins is critically important for their efficacy and safety, and the ability to characterize it at various levels (from sequence to conformation) is critical not only at the quality control stage, but also throughout the discovery and design stages. Biological mass spectrometry (MS) offers a variety of approaches to study structure and behavior of complex protein drugs and has already become a default tool for characterizing the covalent structure of protein therapeutics, including sequence and post-translational modifications. Recently, MS-based methods have also begun enjoying a dramatic growth in popularity as a means to provide information on higher order structure and dynamics of biotechnology products. In particular, several MS-based methods recently developed in our laboratory offer a convenient way to assess the integrity of protein conformation and monitor interactions of protein drugs with their therapeutic targets and other physiological partners using simple model systems. MS-based methods are also applied to study pharmacokinetics of biopharmaceutical products, where they begin to rival traditional immunoassays. MS already provides valuable support to all stages of development of biopharmaceuticals, from discovery to post-approval monitoring, and its impact on the field of biopharmaceutical analysis will undoubtedly continue to grow.

Learn more at www.chem.umass.edu/people/kaltashovlab/

Academic Background

  • Postdoctoral Training: Johns Hopkins University School of Medicine
  • PhD University of Maryland Baltimore County
  • MS Moscow Institute of Physics and Technology
Y. Zhao, R.R. Abzalimov, and I.A. Kaltashov. Interactions of intact unfractionated heparin with its client proteins can be probed directly using native electrospray ionization mass spectrometry. Anal. Chem. 2016, 88, 1711-1718.
J.W. Pawlowski, N. Kellicker, C.E. Bobst, and I.A. Kaltashov. Assessing the iron delivery efficacy of transferrin in clinical samples by native electrospray ionization mass spectrometry. Analyst 2016, 141, 853-861.
I.A. Kaltashov, S.J. Eyles. Mass Spectrometry in Structural Biology and Biophysics: Architecture, Dynamics and Interaction of Biomolecules, 2nd edition. New York: John Wiley & Sons, Inc., 2012 (ISBN 978-0-470-93779-2)
I.A. Kaltashov, C.E. Bobst, R.R. Abzalimov, G. Wang, B. Baykal, S. Wang. Advances and challenges in analytical characterization of biotechnology products: Mass spectrometry-based approaches to study properties and behavior of protein therapeutics. Biotechnol. Adv., 2012, 30, 210-222
C.E. Bobst, S. Wang, W.-C. Shen, and I.A. Kaltashov. Mass spectrometry study of a transferrin-based protein drug reveals the key role of protein aggregation for successful oral delivery. Proc. Natl. Acad. Sci. U.S.A. 2012, 109, 13544-13548
G. Wang, R.R. Abzalimov, I.A. Kaltashov. Direct monitoring of heat-stressed biopolymers with temperature-controlled electrospray ionization mass spectrometry. Anal. Chem. 2011, 83, 2870-2876
I.A. Kaltashov, C.E. Bobst, R.R. Abzalimov, S.A. Berkowitz, D. Houde. Conformation and dynamics of biopharmaceuticals: transition of mass spectrometry-based tools from academe to industry. J. Am. Soc. Mass Spectrom. 2010, 21, 323-337
 
Contact Info

Department of Chemistry
N369 Life Sciences Laboratory
240 Thatcher Way
Amherst, MA 01003-9292

(413) 545-1460
kaltashov@chem.umass.edu

www.chem.umass.edu/people/kaltashovlab/