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Illumina MiSeq Sequencing System

Genomics Resource Laboratory

Announcements

Discounted IDT pricing through UMass Amherst Genomics Resource Lab Portal. Available to all Five College Campuses!

Download PDF for more information.

Genomics Resource Laboratory provides high-throughput next-generation DNA sequencing services to academic and to off-campus researchers at affordable rates.

NGS instrumentation includes Illumina MiSeq, Illumina NextSeq 500, and Fluidigm C1 Single-Cell Analysis System.

GRL also has additional instrumentation required for sample processing and DNA/RNA library preparation for NGS. Instrumentation includes - Diagenode Bioruptor Pico Sonicator, Agilent 2100 Bioanalyzer, Qubit 3.0 Fluorometer, BluePippin (Sage Science) DNA size selection system, FastPrep-24 5G Sample Homogenizer (MP Biomedicals), Stratagene Mx3005P qPCR system, Eppendorf EpMotion 5070 for automated liquid sample dispensing.

GRL maintains Supply Center and Freezer Program located in Morrill 4, N214 (Currently we have Bio-Rad, please see inventory list).

GRL and Integrated DNA Technologies (IDT) have created a portal for Discounted Primer orders.
Visit www.idtdna.com/umamherst for more information

Supporting robotics instrumentation is also available for sample processing.

Additional instrumentation for deep sequencing are expected to come on line will provide UMass researchers and industrial collaborators with capabilities to perform applications from microbial communities to the full human genome. Specific instrumentation will include:

  • Illumina MiSeq System
  • Illumina NextSeq 500 System
  • Fluidigm C1 Single-Cell Analysis System
  • Diagenode Bioruptor Pico Sonicator
  • Agilent 2100 Bioanalyzer
  • Qubit 3.0 Fluorometer
  • BluePippin (Sage Science) DNA Size Selection System
  • FastPrep-24 5G Sample Homogenizer (MP Biomedicals)
    DNA/RNA/Protein isolation
  • Stratagene Mx3005P qPCR System
  • Thermo Scientific Savant DNA120 High-Performance SpeedVac Concentrator
    efficient concentration and drying of small-volume DNA/RNA samples
  • Eppendorf EpMotion 5070
    automated liquid sample dispensing
   
Sequencing Services
Campus Users $2-$6/sample
External Users $2.50-$6.50/sample
Data Fees
Small number of Samples $96/plate
Large number os Samples
(4-10 plates)
$50/plate
Large number os Samples
(>10 plates)
$38/plate
MiSeq System
QPCR Quantification $30/reaction
QC Bioanalyzer $80/reaction
Standard Flow Cell Single Read Full Service $940-$1,790/reaction
Standard Flow Cell Single Read Economy Service
(kit cost not included)
$234-$440/reaction
Standard Flow Cell Paired End Full $940-$1,790/reaction
Standard Flow Cell Paired End Economy Service
(kit cost not included)
$234-$440/reaction
Micro Flow Cell Single Read Full Service $995/reaction
Micro Flow Cell Single Read Economy Service
(kit cost not included)
$245/reaction
Micro Flow Cell Paired End Full $995/reaction
Micro Flow Cell Paired End Economy Service
(kit cost not included)
$245/reaction
Nano Flow Cell Single Read Full Service $725-$840/reaction
Nano Flow Cell Single Read Economy Service
(kit cost not included)
$240-$280/reaction
Nano Flow Cell Paired End Full $725-$840/reaction
Nano Flow Cell Paired End Economy Service
(kit cost not included)
$240-$280/reaction
Rates are subject to change, contact facility to verify current fees.
Updated March 13, 2017
Services

Bio-Rad Freezer Program (product stock list)

Volunteer Position

The Genomics Research Laboratory at Institute for Applied Life Sciences, is looking for volunteer(s) to assist with day to day routine lab activities, managing inventory and instruments, assisting with protocol development (schedule and experience permitting). Ideal for students who are looking forward to pursue research. For more information visit www.umass.edu/ials/genomics

Requirements: Undergraduate (freshman) preferred with biology back ground. Flexible lab hours. Lab hours can be coordinated with class schedule. Please note this is temporary and an unpaid position. Interested candidates please send CV at ranjan@umass.edu with subject heading: GRL_Volunteer_(your name)

For further information contact
Ravi Ranjan, PhD,
Director, Genomics Research Laboratory
Morrill 1, N330
Phone Office: 413-577-4501
Email: ranjan@umass.edu

Facility Staff

Ravi Ranjan
Director/Senior Research Fellow
ranjan@umass.edu
Genomics facility email - genomics@umass.edu
Office:  (413) 577-4501
Lab:  (413) 577-4502

Location

N330 Morrill I
University of Massachusetts Amherst
637 North Pleasant Street
Amherst, MA 01003

Centers