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Illumina MiSeq Sequencing System

Genomics Resource Laboratory

Enables next-generation sequencing capabilities, with a short turn-around time for data production. The Genomics Resource Laboratory, supports next generation sequencing including Illumina MiSeq and NextSeq machines, with capacity to sequence a human genome in less than a day. We also have necessary equipment for library preparation, including an Agilent Bioanalyzer and a Qubit. The GRL strives to provide affordable options to off-campus commercial operations or academic cores providing similar services.

Supporting robotics instrumentation is also available for sample processing.

Additional instrumentation for deep sequencing are expected to come on line will provide UMass researchers and industrial collaborators with capabilities to perform applications from microbial communities to the full human genome. Specific instrumentation will include:

  • Agilent Bioanalyzer (sample and NGS library QC)
  • MiSeq Gene and Small Genome Sequencer: a desktop genome analyzer capable of microbial genomics and rapid targeted sequencing (i.e. faster, smaller scale)
  • NextSeq Sequencer: a genome analyzer capable of human whole genome sequencing and plant transcriptomics (i.e. medium speed, large scale)
   
Sequencing Services
Campus Users $2-$6/sample
External Users $2.50-$6.50/sample
Data Fees
Small number of Samples $96/plate
Large number os Samples
(4-10 plates)
$50/plate
Large number os Samples
(>10 plates)
$38/plate
MiSeq System
QPCR Quantification $30/reaction
QC Bioanalyzer $80/reaction
Standard Flow Cell Single Read Full Service $940-$1,790/reaction
Standard Flow Cell Single Read Economy Service
(kit cost not included)
$234-$440/reaction
Standard Flow Cell Paired End Full $940-$1,790/reaction
Standard Flow Cell Paired End Economy Service
(kit cost not included)
$234-$440/reaction
Micro Flow Cell Single Read Full Service $995/reaction
Micro Flow Cell Single Read Economy Service
(kit cost not included)
$245/reaction
Micro Flow Cell Paired End Full $995/reaction
Micro Flow Cell Paired End Economy Service
(kit cost not included)
$245/reaction
Nano Flow Cell Single Read Full Service $725-$840/reaction
Nano Flow Cell Single Read Economy Service
(kit cost not included)
$240-$280/reaction
Nano Flow Cell Paired End Full $725-$840/reaction
Nano Flow Cell Paired End Economy Service
(kit cost not included)
$240-$280/reaction
Rates are subject to change, contact facility to verify current fees.
Updated March 13, 2017

Volunteer Position

The Genomics Research Laboratory at Institute for Applied Life Sciences, is looking for volunteer(s) to assist with day to day routine lab activities, managing inventory and instruments, assisting with protocol development (schedule and experience permitting). Ideal for students who are looking forward to pursue research. For more information visit www.umass.edu/ials/genomics

Requirements: Undergraduate (freshman) preferred with biology back ground. Flexible lab hours. Lab hours can be coordinated with class schedule. Please note this is temporary and an unpaid position. Interested candidates please send CV at ranjan@umass.edu with subject heading: GRL_Volunteer_(your name)

For further information contact
Ravi Ranjan, PhD,
Director, Genomics Research Laboratory
Morrill 1, N330
Phone Office: 413-577-4501
Email: ranjan@umass.edu

Facility Staff

Ravi Ranjan, Director
ranjan@umass.edu
(413) 577-4501
 

Morrill I, N330B

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