Using enzymes to build, break and read hybrid DNA nanomaterials: from nanoscale self-assembly to intracellular gene regulation

CHEM Seminar
Jessica Rouge
Univ. of Connecticut
Lederle Graduate Research Tower 1634
Reception and light refreshments at 11:00 a.m.
Biology has evolved the quintessential nanoscale assembly of nucleic acids, lipids and proteins in the form of a virus. Viruses are built from self-assembled peptide subunits surrounding charged nucleic acids, packaged within a lipid-like envelope. Viral coat proteins are enzymatically degraded, in a location-specific manner, releasing contents into the surrounding environment. Our lab seeks to mimic not only the assembly, but the programmed disassembly, of biomolecule-based nanomaterials through a combination of chemical crosslinking strategies and enzymatic assembly steps. Using a hybrid DNA surfactant and a peptide-based self-assembly method, we have built a series of crosslinked micelle systems that breakdown in response to the presence of various stimuli, including pH and enzymes. The nanocapsule displays highly specific responses in the presence of closely related proteases and can be modified with inorganic nanoparticles for monitoring its stability using electron microscopy. Integrating natural biochemical ques into the assembly and degradation pathways of nanomaterials brings us one step closer to designing precision biomaterials, paving the road for greater accuracy in applications ranging from gene delivery to biosensing.
Speaker: Faculty Host: Mingxu YouEvent Contact:
Laura Sedberrylsedberry@chem.umass.edu
(413) 545-2585

Thursday, September 26, 2019 - 11:30am