Enables the production, separation, and isolation of biomolecules (e.g., proteins, natural products, cells) for research, commercial, and clinical purposes. The ability to cultivate a variety of cell types (microbial, animal, plant) and optimize process parameters to enable production at the scale of interest is important for protein science and engineering, polymer science and engineering, cellular engineering, and bioprocess development. This facility will enable IALS research in areas such as antibody production, protein-polymer conjugates, metabolites, microbial consortia, protein-based pharmaceuticals, anaerobic fermentation, and biofilm formation. The facility will house instruments at a variety of scales, enabling parallel high-throughput culturing for optimizing conditions and mid-scale production for synthesizing larger quantities of production of interest. Protein separations units will also be available to enable recovery of biomolecules of interest.
This facility will offer equipment to perform cell culture investigations, housing both a New Brunswick BioFlo III controlled fermentation system and Hel Ltd. BioXplorer four chamber bioreactor that offers the capability of running simultaneous cultures under different conditions. In addition to the bioreactors, the laboratory houses basic items needed for microbial culture including laminar flow hood, autoclave, incubator shakers, and research-grade microscopes. A YSI biochemistry analyzer is available for off-line measurements of extracellular species concentrations.
The instruments in this facility enable both production and separation of biomolecules of interest to IALS research groups. Instruments are listed below. All equipment will be housed in a single cohesive space in LSL South.
- MiniBioreactor: This 16 x 500 mL bioreactor to support the cultivation of both microbial and higher eukaryotic cells. These individual reactors can run in parallel and under strictly controlled conditions (e.g., oxygen supply, pH, agitation rate, temperature, etc.) and can therefore be used to screen conditions for optimal culture performance. Data from such studies can be used to inform larger scale culture design and future experimentation.
- Micro-Matrix 24-well Bioreactor: This small scale system (5 mL culture per well) enables high-throughput screening of cultures/isolates for particular functions (i.e., novel or specified activities) as well as identification of optimal culturing conditions. The ability to run 24 parallel cultures while utilizing only a small amount of biomass is particularly useful for higher eukaryotic cell systems.
- PhotoBio Autoclavable Bioreactor (2): These 3L bioreactor systems enables mid-scale production of biomolecules from both microbial and higher eukaryotic cells. This equipment will arm investigators with the capability of developing scalable bioprocesses as well as single use fermentations/cell cultures for production of research quantities of proteins for further analyses.
- ATKA Chromatography Systems (2): These liquid chromatography units enable protein purification at the research laboratory scale and will serve as the core protein separations equipment in the facility.